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Asian-Australas J Anim Sci > Volume 26(10); 2013 > Article
Huo, Zhu, and Mao: Effects of Feeding Increasing Proportions of Corn Grain on Concentration of Lipopolysaccharide in the Rumen Fluid and the Subsequent Alterations in Immune Responses in Goats


This study was conducted to investigate the effects of feeding increasing proportions of corn grain on concentration of lipopolysaccharide (LPS) in the rumen fluid and the subsequent alterations in immune responses as reflected by plasma concentrations of serum amyloid A (SAA) and haptoglobin (Hp) in goats. Nine goats were assigned to three diets (0%, 25%, and 50% corn grain) in a 3 ×3 Latin square experimental design. The results showed that as the proportion of dietary corn increased, the ruminal pH decreased (p< 0.001), and the concentrations of propionate (p<0.001), butyrate (p<0.001), lactic acid (p = 0.013) and total volatile fatty acid (p = 0.031) elevated and the ruminal LPS level increased (p<0.001). As the proportion of dietary corn increased, the concentration of SAA increased (p = 0.013). LPS was detectable in the blood of individual goats fed 25% and 50% corn. A real-time PCR analysis showed that the copy number of phylum Bacteroidetes (p<0.001) was reduced (4.61×109copies/mL to 1.48×109copies/mL) by the increasing dietary corn, and a correlation analysis revealed a significant negative correlation between the number of Bacteroidetes and rumen LPS levels. Collectively, these results indicated that feeding goats high proportions (50%) of corn grain decreased the ruminal pH, increased LPS in the rumen fluid and tended to stimulate an inflammatory response.


It is a common practice to feed ruminant animals amount of concentrate to accelerate milk production and body weight gain. However, feeding diets high in readily fermentable carbohydrates increases the odds of developing subacute ruminal acidosis (SARA) and decreases the long-term productive performance of beef and milk (Stone, 2004). In addition, numerous studies have recently demonstrated that feeding cattle diets rich in highly degradable carbohydrates results in disorders such as acidosis, fatty liver, laminitis, liver abscesses, displaced abomasum, and bloat (Nocek, 1997; Ametaj et al., 2005). Although the exact mechanisms underlying the relationship between feeding large amounts of cereal grains and the high incidence of metabolic diseases is not yet completely understood, research has described several events associated with feeding high grain diets. For example, digestion of increasing amounts of cereal grains was shown to initiate major changes in the composition of microbiota in favor of Gram-negative bacteria (Dunlop, 1972). Additionally, other researchers found that bacterial alterations were associated with the release of large amounts of lipopolysaccharide (LPS), a cell-wall component of all Gram-negative bacteria, in the rumen fluid (Nagaraja et al., 1978; Andersen et al., 1994; Gozho et al., 2006; Li et al., 2012). This increase in luminal LPS could increase rumen permeability, lead to the translocation of LPS or other immunogenic compounds into circulation, and trigger systemic inflammatory response (Ametaj et al., 2007; Khafipour et al., 2009).
Worldwide, the goat population is approximately 909 million (FAO: 2010, Yearbook statistics. Available at: http://faostat.fao.org/site/573/default.aspx#ancor). In the last 20 yrs, the goat population increased 53.88% worldwide. In China, goat production is the largest production among ruminants. This may be because goats have stronger roughage resistance and stress resistance than cows and sheep, considering that China is short of good quality of forages and consequently large amount of roughages and grains are used for ruminants. In addition, despite previous researches have demonstrated that high grain feeding increases the concentrations of the acute phase proteins serum amyloid A (SAA), and haptoglobin (Hp), which are markers of inflammation, in peripheral blood of cattle and sheep (Gozho et al., 2005; 2007; González et al., 2008, Nazifi et al., 2009), to our knowledge, little information is available on the concentration of LPS in the rumen fluid and the subsequent alterations in immune responses during high grain feeding in goats.
We hypothesised that feeding high proportions of corn grain could increase the ruminal LPS concentration, ultimately resulting in changes in SAA or Hp in peripheral blood in goats. Therefore, the main objective of this study was to determine if feeding high proportions of corn grain could result in increases in the concentration of ruminal LPS and stimulate an inflammatory response in goats, in addition, the effects of high grain feeding on changes in the composition of rumen bacterial community were also determined.



All of the management, ethical and experimental procedures were conducted according to the Guidelines for the Care and Use of Animals at Nanjing Agricultural University, 1999.

Animals and diets

A total of nine male goats (25±3 kg) fitted with ruminal cannulas were used in this study in a 3×3 Latin square design. The each experimental period was 21 d, with the first 14 d used for diet adaptation and the last 7 d used for measurements. The animals were housed in individual pens with free access to water and were fed twice daily at 08:00 h or 20:00 h. Dry matter intake was 600 g/d/head. To challenge the goats with graded amounts of rumen fermentable carbohydrates, the basic ration was 0, 25, or 50% (DM) corn grain to provide three different dietary treatments. All of the diets were formulated to meet the nutrient requirements of a 25 kg meat goat as per NRC (2007) guidelines (Table 1).

Sample collection

Samples of the rumen contents (approx, 50 mL) were obtained on d 1, 3, 5, and 7 of the measurement day shortly before the morning feeding (07:45 h) and at 2 h, 4 h and 8 h after feeding. All of the rumen content samples were collected through the rumen cannula into a 140 mL plastic container. According to Mao et al. (2008), 3 mL of the rumen contents collected on d 7 of measurement day before the morning feeding (07:45 h) was used for DNA extraction (Mao et al., 2008). The other rumen samples were strained through four layers of cheesecloth, and the pH of the rumen fluid was measured immediately after collection using a mobile pH meter. A 2 mL aliquot of freshly prepared 25% (250 mL/L) meta-phosphoric acid was added to 8 mL of strained ruminal fluid; the mixture was centrifuged (17,000 g for 10 min), and the supernatant fluid was stored at −20°C for determination of the volatile fatty acids (VFA). A 5 mL sample of the rumen fluid was used for the LPS content measurement, and the remaining rumen fluid samples were stored at −20°C for the lactic acid analysis. Starting at 08:00 on d 7, samples of ruminal fluid were collected every 2 h up to 20:00 to investigate the diurnal responses of the pH.
Blood samples were obtained from the jugular vein on d 7 of the measurement period at 07:45 h, shortly before the 08:00 h morning feeding, and at 4 h and 8 h after feeding. Sodium-heparinised, pyrogen-free, 10 mL vacuum glass tubes were used to collect the blood samples. Immediately after collection, the blood samples were stored on ice, and the plasma was separated within 20 min of withdrawal by centrifuging at 3,000 g at 4°C for 20 min. A portion of the plasma was transferred to pyrogen-free glass tubes and stored at −20°C until the LPS was analysis. The remaining plasma was divided into aliquots of 2 mL and stored at −20°C until analysis for SAA and Hp.

Extraction of genomic DNA

The DNA was extracted according to a bead-beating method using a mini-bead beater (Biospec Products), followed by phenol-chloroform extraction (Mao et al., 2008). The solution was then precipitated with isopropanol, and the pellets were suspended in 50 μL Tris ethylene diamine tetraacetic acid (TE). The DNA samples were quantified using a Nanodrop spectrophotometer (Nyxor Biotech).

Real-time PCR assay for the quantification of the Firmicutes and Bacteroidetes

Real-time PCR was performed using an Applied Biosystems 7300 Real-Time system (Applied Biosystems) with phylum Firmicutes and Bacteroidetes primers (Guo et al., 2008). The reaction mixture (20 μL) consisted of 10 μL IQ SYBR Green Supermix (Bio-Rad), 0.2 M each oligonucleotide primer set and 1 μL template DNA. The measurements were performed in triplicate for each run, including a negative control. The amount of DNA in each sample was spectrophotometrically determined in triplicate using a GeneQuant pro RNA/DNA calculator (Amersham Biosciences), and the mean values were calculated. The DNA concentration of the PCR product measured using the GeneQuant pro RNA/DNA calculator can be directly related to the copy number using the following equation: copy number/μL = (C/X)×0.912×1012, where C is the DNA measured concentration (ng/μL) and X is the PCR fragment or plasmid length (base bp/copy). PCR was performed using one cycle of 95°C for 2 min, then 40 cycles at 95°C for 15 s and 60°C for 1 min (Denman and McSweeney, 2006).

Chemicals analysis

Samples of the concentrate, Chinese wildrye (Leymus chinensis) and alfalfa hay were collected during the data collection week, ground to pass through a 1 mm screen and analysed for dry matter (DM) (method 930.15; AOAC, 1995), ash (method 942.05; AOAC, 1995), ether extract (method 920.39; AOAC, 1995), neutral detergent fibre (NDF; Van Soest et al., 1991), The crude protein (CP) (N×6.25) was determined by the method of Krishnamoorthy et al. (1982), and the VFA in the ruminal fluid were measured using a capillary column gas chromatograph (Shimadzu, GC-14A) (Mao et al., 2008). The lactic acid concentration was analysed following the method described by Baker and Summerson (1941).

Rumen LPS analysis

As described by Emmanuel et al. (2008), the concentration of cell-free LPS in the rumen fluid supernatant was determined by the Limulus amoebocyte lysate (LAL) assay (Xiamen Houshiji, Ltd., Xiamen, China). Five millilitres of rumen fluid were centrifuged at 10,000 g for 45 min at 4°C, and the supernatant was aspirated gently to prevent its mixing with the pellet and passed through a disposable 0.22 μm sterile, pyrogen-free filter (Fisher Scientific). The filtrate was collected in pyrogen-free glass tubes and heated at 100°C for 30 min. The samples were cooled at room temperature (20°C) for 10 min and stored at −20°C until analysis. For the assay, the rumen fluid was diluted 10,000-fold using the LAL reagent water and pyrogen-free test tubes (Xiamen Houshiji, Ltd., Xiamen, China). The method and the quantities of the reagents described in the kit were modified to produce greater standard ranges of 0.01 to 10 endotoxin units (EU)/mL. A control standard LPS, containing 10 EU endotoxin per vial, was used to prepare the standard solutions. All of the samples were tested in duplicate, and the optical density values were measured using a microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation), at a wavelength of 405 nm.

Plasma LPS analysis

As described by Khafipour et al. (2009a), the concentration of LPS in the plasma was determined by the LAL assay (Xiamen Houshiji, Ltd., Xiamen, China) with a minimum detection limit of 0.01 EU/mL. Before the LAL assay, the samples were pretreated as described by Khafipour et al. (2009a). Frozen plasma samples were thawed at 37°C and vortexed, then 100 μL of each sample was diluted at least 10-fold with pyrogen-free water. The diluted samples were incubated at 37°C for 30 min, heated at 75°C for 15 min and cooled to room temperature (19°C) for 45 min. The LAL assay was performed using a 96 well microplate according to the manufacturer’s instructions. All of the samples were tested in duplicate, and the optical density values were measured using a microplate spectrophotometer (Spectramax 190, Molecular Devices Corporation) at a wavelength of 405 nm. The results were accepted when the intra-assay coefficient of variation was <10%. The minimum detection level of LPS in the plasma was 0.01 EU/mL using this method.

Plasma acute-phase proteins

The plasma concentrations of Hp and SAA were determined using ELISA kits (TP-801, and TP-802, respectively, Tri-Delta Diagnostics), as described by Gozho et al. (2005). The samples were initially diluted 1:5 for Hp and 1:500 for SAA and assayed according to the manufacturer’s instructions. The samples were analysed in duplicate, and the absorbance values were measured at 630 nm for Hp and 450 nm for SAA using a microplate spectrophotometer (Spectra Max 340 PC, Molecular Devices Corp.).

Data analysis

To test the influence of the diet on the lactic acid, VFA, LPS, rumen pH, SAA and Hp concentrations, the MIXED procedure was performed using the diet, period and day as fixed effects and the goats as a random effect. The samples collected at different times for the same goat were considered as repeated measures in the analysis of variance (ANOVA). Within the model, the differences of the least squares means were compared using a Bonferroni t test only after for a significant effect was found for the diet. The data were compiled into the means±standard errors of the mean (SEM) using PASW Statistics 18 (SPSS), and the level of significance was set at p<0.05.
For the ruminal bacteria community parameters, the general linear model (GLM) procedure was performed with diet and period as fixed effects. The data were compiled into the means±SEM using PASW Statistics 18 (SPSS), and the level of significance was set at p<0.05.
Correlations between each microbial dataset and the LPS levels were assessed using Pearson’s correlation test with GraphPad Prism (5.00). Significance was declared at p<0.05.


Effect of a high grain diet on the diurnal rumen pH

The diurnal data indicated that the goats fed the 0% corn grain diet had a higher ruminal pH compared with the 25% and 50% corn groups (p<0.001) (Figure 1). The ruminal pH also varied over the time during the day (p<0.001). In general, the ruminal pH in all of the goats declined during the first few hours after the morning feeding, regardless of the diet used. However, during the time period from almost 1 h after feeding until the sampling at 12 h, the average ruminal pH was <5.8 in the 50% corn group and remained significantly lower than that of the 0% corn group; the duration for a ruminal pH below 5.8 was approximately 10 h after the first feeding in the 50% corn group.

Effect of a high grain diet on the rumen pH, rumen fluid composition and free LPS

The rumen pH, rumen fluid composition and LPS concentration are presented in Table 2. As the proportion of dietary corn increased, the rumen pH decreased (p<0.001), the propionate (p<0.001), butyrate (p<0.001), isobutyrate (p = 0.079), valerate (p<0.001), isovalerate (p<0.001), total volatile fatty acid (TVFA) (p<0.031) and lactic acid (p = 0.013) concentrations increased. The LPS concentration in the rumen liquid increased (p<0.001) as the proportion of dietary corn increased.

Effects of a high grain diet on the concentrations of serum amyloid A and haptoglobin in peripheral blood

Changes in the concentrations of SAA and Hp in the peripheral blood are shown in Table 3. As the proportion of dietary corn increased, the data indicated potential increases in the concentrations of Hp (p = 0.152) in the blood, and the concentration of SAA increased significantly (p = 0.013). LPS was detected in the peripheral blood in three of nine goats in the 25% corn group (0.406, 0.846 and 0.241 EU/mL, respectively, data not shown) and 50% corn group (0.243, 0.126, and 1.47 EU/mL, respectively, data not shown). In the control, however, LPS was not detected (Table 3).

Effect of a high grain diet on the 16s rRNA gene copy number of Firmicutes and Bacteroidetes and its relationship with the rumen LPS levels

The real-time PCR showed that, as the proportion of dietary corn increased, the copy number of phylum Bacteroidetes decreased (p<0.001), and the ratio of Firmicutes to Bacteroidetes increased (p = 0.001) (Table 4). In contrast, no significant difference was observed in the number of Firmicutes (p = 0.146) in the 25% and 50% groups compared with the control.
To assess the possible relationship between the bacterial abundance and the LPS level, a correlation analysis was conducted on the two most commonly represented phyla and the LPS concentration. The results showed a significant negative correlation between the abundance of Bacteroidetes and LPS levels (R = −0.437, p = 0.023) (Figure 2a), whereas no significant correlation was observed between the abundance of Firmicutes and LPS levels (R = −0.085, p = 0.665) (Figure 2b).


The pH of the rumen is a critical factor in the normal and stable function of the rumen because of its profound effect on microbial populations and fermentation and on the physiological functions of the rumen, mainly the motility and absorptive functions. The ruminal pH can be influenced by the intake of fermentable carbohydrate, and the rates of utilisation and absorption of acids (Nagaraja and Titgemeyer, 2007). The diet in our study containing a high proportion grain was rich in rapidly fermentable carbohydrates, resulting in the decrease in the rumen pH to acidotic values, with the rumen fluid pH being below 5.8 for more than 10 h/d in the 50% corn group. Recent studies suggested that duration of episodes during which ruminal pH remains below 5.8 for longer than 5.4 h/d was safer indicators of SARA (Gozho et al., 2007; Zebeli et al., 2008). Thus, based on this definition, the SARA was induced by the 50% corn grain feeding in this study. On the other hand, we also found, as the proportion of dietary corn increased, the propionate, butyrate, isobutyric acid, valerate, isovalerate and TVFA concentrations increased, while the ruminal lactic acid concentration was low, not exceeding 0.5 mM. The generally low lactic acid concentration suggests that an elevated total VFA concentration is the main cause of a low ruminal pH.
Results are in agreement with our working hypothesis that feeding increasing proportions of corn grain to goats was associated with increased concentrations of LPS in the rumen fluid. Feeding diets containing 25 and 50% corn grain resulted in higher amounts of free LPS in the rumen fluid compared with those of goats receiving 0% corn grain. Our findings are consistent with previous studies demonstrating enhanced content of LPS in the rumen fluid from feedlot or dairy cattle fed diets containing high proportions of grain (Andersen, 2003; Emmanuel et al., 2008; Li et al., 2012). Gozho et al. (2006) reported a 3-fold increase in the concentration of LPS in the rumen fluid when steers were moved from an all-forage diet to a 61% concentrate diet. Interestingly, they failed to detect any changes in the amount of LPS in the rumen from an all-forage to 41% concentrate diet. In our study, an increase was observed when the amount of corn grain was enhanced from 0 to 25 or 50%, further, an about 7-fold increase in the concentration of LPS in the rumen fluid when goats were fed the 50% grain compared with the 0% control. The difference in results might be related to the difference in the relative amount of grain fed to steers vs. goats, as compared with the steers, goats are fed greater amounts of grain (0.014 kg/body weight vs 0.0075 kg/body weight).
Two most abundant bacterial phyla that inhabit the rumen are Gram-positive Firmicutes and the Gram-negative Bacteroidetes (Khafipour et al., 2009b; Fernando et al., 2010), and it was known that LPS is a cellular component of Gram-negative bacteria (Andersen, 2003; Emmanuel et al., 2008). Plaizier et al. (2008) reported there is an increase in the concentration of LPS in the rumen in both acute and subacute acidosis. Khafipour et al. (2009b) observed a decrease in the number of Bacteroidetes in the rumen as a consequence of a high grain diet. In the present study, the increase in corn grain significantly increased the concentration of LPS in the rumen and decreased the number of Bacteroidetes; furthermore, the correlation analysis showed a negative correlation between the copy number of Bacteroidetes and the ruminal LPS levels. This association might be linked to the high cell autolysis rate of the Gram-negative Bacteroidetes species when accumulating toxic glucose fermentation end products during high grain feeding (Wells and Russell, 1996).
Previous studies reported that luminal LPS can be translocated into the systemic circulation when the concentration of LPS in the lumen of the gastrointestinal tract increased after being fed a high grain diet (Emmanuel et al., 2008; Khafipour et al., 2009a) or the oral administration of large doses of LPS (Aschenbach et al., 2003). It has been suggested that the mechanism of LPS translocation involves an impairment of the barrier function of the rumen epithelium due to the high luminal osmolality, acidic pH and high concentrations of LPS, which can cause swelling and rupture of ruminal papillae with high grain-based diets (Kleen et al., 2003; Emmanuel et al., 2007). In the present study, the concentration of LPS in peripheral blood was undetectable in all of the goats fed the 0% grain groups, but it was detectable in some of the goats fed the 25% and 50% grain groups, in spite of the fact that the statistical analysis indicated that the concentrations of LPS in the peripheral blood in the 25% and 50% grain groups were not different from the control (Table 3). These results indicated that an impairment of the barrier function of the rumen epithelium might occur in goats fed a high corn grain diet. However, the fact that LPS became detectable in the blood in some of the goats, but not in others, in the 25% and 50% grain groups may be partly explained by the difference between the individual ruminants (Zebeli et al., 2012). In the dairy industry, it is known that the risk for acidosis is not the same for all cows (Beauchemin and Penner, 2009): individual dairy cows exhibit tremendous variation in the degree of acidosis they experience, even when fed and managed similarly (Beauchemin and Penner, 2009). The variation in the degree of acidosis in the rumen may result from a difference in the absorptive functions in individual ruminants and may result in differences in the permeability of the ruminal mucosal tissue. Penner et al. (2009) provided evidence that differences in the absorptive capacity of the ruminal epithelia between individual sheep accounted for a large variation in the ruminal pH among animals. Chen et al. (2012) showed that there are differences in rumen bacterial diversity and density of both rumen content and epithelium communities between rumen acidosis-susceptible (AS) and acidosis-resistant steers, and a higher bacterial density was detected in AS animals. Therefore, in the present study, given that there is some variability in preventing the LPS translocation in individual goats, it seems reasonable that LPS was detectable in some goats, but not in others, with the high grain diet.
Recent research has shown that feeding high grain diets enhances the concentrations of SAA in the blood of both dairy (Emmanuel, 2008) and beef cattle (Ametaj, 2009) but that the plasma Hp is not affected. The reason for the increased SAA in the plasma of cattle fed high grain diets is suggested to be related to the translocation of LPS into the systemic circulation, which then stimulates the release of such pro-inflammatory cytokines, such as tumour necrosis factor-α, interleukin (IL)-1 and IL-6 by liver macrophages (Gabay and Kushner, 1999; Ametaj et al., 2011). In our study, the concentration of SAA was increased significantly as the proportion of dietary corn increased, whereas the Hp levels were not affected, in agreement with reports by Emmanuel (2008) and Ametaj (2009). However, as discussed previously, as the proportion of dietary corn increased, in the current study, LPS became detectable in the blood in some of goats but not in others. The fact that the high grain diet stimulated an inflammatory response, as indicated by increased SAA, in all of the goats but that LPS was only found in the blood of some goats fed the 25% and 50% corn concentrate, suggested that the absorbed rumen LPS might not be the only inducer of an acute-phase response with a high grain diet. Previous studies demonstrated that other bacterial immunogens, such as biogenic amine, and Escherichia coli also correlated with an inflammatory response during SARA (Underwood, 1992; Hayward et al., 2006). Therefore, the mechanism regarding the relationship between the inflammation responses and the inflammation stimuli with a high grain diet need to be further evaluated.


Overall, the results of this study indicate that feeding increasing proportions of corn grain in the diet led to a decreased rumen pH, enhanced concentration of LPS in the rumen fluid, enhanced concentrations of plasma acute-phase proteins, such as SAA, and a lower number of Bacteroidetes. Accumulated rumen free LPS could translocate to the bloodstream with a grain diet, but the level of LPS in the peripheral blood varied between individuals. Further research is warranted to gain a better understanding of the relationship between the inflammation responses and all of the abnormal metabolites in the rumen when feeding different grain concentrate diets.


This work was supported by Natural Science Foundation of China (NSFC) (31172228) and Fundamental Research Funds for the Central Universities (KYZ201114).


Conflict of interest statement

None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of this report.

Figure 1.
The daily rumen pH variation during the 12 h after morning feeding (n = 9).
Figure 2.
Correlations between the copy numbers of rumen Firmicutes (a) or Bacteroidetes (b) and the rumen LPS concentration.
Table 1.
Composition and ingredients of experimental diets
Item Corn grain proportion (% of diet dry matter)
0 25 50
Ingredient,% of DM
  Corn grain 0 25 50
  Soy bean meal 8 8 8
  Alfalfa 10 6 3
  Chinese wildrye 80 59 37
  Dicalcium phosphate 0.5 0.5 0.5
  Salt 0.5 0.5 0.5
  Mineral and vitamin premixa 1 1 1
Nutrient composition (% DM)
  Dry matter 94.81 92.4 90.94
  Organic matter 92.47 93.37 94.86
  Crude protein 10.08 10.50 10.66
  Ether extract 1.93 2.56 2.76
  Neutral detergent fibre (NDF) 36.76 32.36 26.76
  Calcium 0.60 0.48 0.38
  Phosphorus 0.09 0.11 0.14
  Metabolizable energy (MJ/kg DM) 8.09 8.93 9.87

a Contained 7 g/kg of Fe, 8 g/kg of Zn, 5 g/kg of Mn, 1.2 g/kg of Cu, 130 mg/kg of I, 27 mg/kg of Se, 45 mg/kg of Co, 1,100,000 IU/kg of vitamin A, 300,000 IU/kg of vitamin D, and 3,200 IU of vitamin E.

Table 2.
Rumen pH, rumen fluid composition and LPS concentration
Corn grain proportion (% DM)
SEM p value
0 25 50
Ruminal pH 6.28a 5.93b 5.65c 0.039 0.018
Acetate (mmol/L) 62.73 60.49 59.49 0.960 0.053
Propionate (mmol/L) 12.31b 12.77b 14.11a 0.244 <0.001
Isobutyrate (mmol/L) 1.48b 1.63a 1.65a 0.058 0.079
Butyrate (mmol/L) 5.35b 8.79a 10.71a 0.180 <0.001
Isovalerate (mmol/L) 0.49c 0.62b 0.71a 0.044 <0.001
Valerate (mmol/L) 0.96b 1.29a 1.46a 0.019 <0.001
TVFA (mmol/L) 83.31c 85.61b 88.12a 1.284 0.031
The ratio of acetate to propionate 5.18a 4.81b 4.43b 0.078 <0.001
Lactic acid (mmol/L) 0.29b 0.35ab 0.44a 0.035 0.013
LPS concentration in the rumen liquid (EU/mL) 5,316c 21,075b 35,116a 6,135 <0.001

a,b,c The means within a row with different superscripts differ significantly (p<0.05).

Table 3.
The SAA, Hp and LPS concentrations in blood
Corn grain proportion (% DM)
SEM p value
0 25 50
SAA (μg/mL) 13.614b 16.367a 15.317ab 2.03 0.013
Hp (μg/mL) 353.271 402.423 384.845 45.11 0.152
LPS (EU/mL) ND 0.166 0.204 0.131 0.402

ND = Not detected.

a,b The means within a row with different superscripts differ significantly (p<0.05).

Table 4.
The 16s rRNA gene copies of phylum Firmicutes and Bacteroidetes in rumen
Item Corn grain proportion (% of DM)
SEM p value
0 25 50
Firmicutes (×108copies/mL) 9.56 6.43 6.17 1.289 0.146
Bacteroidetes (×109copies/mL) 4.61a 1.95b 1.48b 0.391 <0.001
Firmicutes/bacteroidetes 0.21b 0.32a 0.40a 0.033 0.001

a,b The means within a row with different superscripts differ significantly (p<0.05).


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