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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0384    [Accepted] Published online October 13, 2020.
Zearalenone regulates key factors of the Keap1-Nrf2 signaling pathway in duodenum of post-weaning gilts
Qun Cheng1  , Shu zhen Jiang1  , Li bo Huang1  , Wei ren Yang1,*  , Zai bin Yang1 
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Department of Animal Sciences and Technology, Shandong Agricultural University, Taian 271018, Shandong, China
Correspondence:  Wei ren Yang, Tel: +86 18605489796, Fax: +86 0538 8249371, Email: wryang@sdau.edu.cn
Received: 2 June 2020   • Revised: 15 September 2020   • Accepted: 25 September 2020
Abstract
Objective
This study explored the mechanism of the Kelch-like erythroid cell-derived protein with CNC homology-associated protein 1 (Keap1)–nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathway under conditions of ZEA-induced oxidative stress in the duodenum of post-weaning gilts.
Methods
Forty post-weaning gilts were randomly allocated to four groups and fed diets supplemented with 0, 0.5, 1.0, or 1.5 mg/kg ZEA.
Results
The results showed significant reductions in the activity of the antioxidant enzymes total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-Px), and increases the malondialdehyde (MDA) content with increasing concentrations of dietary ZEA. Immunohistochemical analysis supported these findings by showing a significantly increased expression of Nrf2 and glutathione peroxidase 1 (GPX1) with increasing concentrations of ZEA. The relative mRNA and protein expression of Nrf2, GPX1 increased linearly (p<0.05) and quadratically (p<0.05), which was consistent with the immunohistochemical results. The relative mRNA expression of Keap1 decreased linearly (p<0.05) and quadratically (p<0.05) in the duodenum as the ZEA concentration increased in the diet. The relative mRNA expression of modifier subunit of glutamate-cysteine ligase (GCLM) increased quadratically (p<0.05) in all ZEA treatment groups and the relative mRNA expression of quinone oxidoreductase 1 (NQO1) catalytic subunit of glutamate-cysteine ligase (GCLC) decreased linearly (p<0.05) and quadratically (p<0.05) in the ZEA1.0 group and ZEA1.5 group. The relative protein expression of Keap1 and GCLM decreased quadratically (p<0.05) in the duodenum as the ZEA concentration increased in the diet, respectively. The relative protein expression of NQO1 increased linearly (p<0.05) and quadratically (p<0.05) in all ZEA treatment groups in the duodenum.
Conclusion
These findings suggest that ZEA regulates the expression of key factors of the Keap1-Nrf2 signaling pathway in the duodenum, which enables resistance to ZEA-induced oxidative stress. Further studies are needed to examine the effects of ZEA-induced oxidative stress on other tissues and organs in post-weaning gilts.
Keywords: Antioxidant; Duodenum; Keap1-Nrf2 Signaling Pathway; Oxidative Stress; Zearalenone


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