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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0392    [Accepted] Published online October 13, 2020.
miR-458b-5p Regulates Ovarian Granulosa Cells Proliferation Through Wnt/β‐catenin Signaling Pathway by Targeting CTNNB1
Wenwen Wang1  , Shen Zhang1  , Qin Zhang1  , Hui Tang1,* 
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, Shandong Province, 271018, China
Correspondence:  Hui Tang, Tel: (+86)18653824661, Fax: (+86)18653824661, Email: tanghui@sdau.edu.cn
Received: 5 June 2020   • Revised: 1 July 2020   • Accepted: 13 September 2020
Abstract
Objective
Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear.
Methods
The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-Catenin pathway. The Cell Counting Kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and Annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the mRNA and protein expression levels.
Results
We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells (TCs) of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule (CD44) and matrix metallopeptidase 7 (MMP7), which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation.
Conclusion
Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development.
Keywords: miR-458b-5p; CTNNB1; Granulosa Cells; Proliferation; Wnt/β-Catenin Signaling Pathway
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