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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0342    [Accepted] Published online October 13, 2020.
Integrated analysis of transcriptomic and proteomic analyses reveals different metabolic patterns in the livers of Tibetan and Yorkshire pigs
Mengqi Duan1  , Zhenmei Wang1  , Xinying Guo1  , Kejun Wang3  , Siyuan Liu1  , Bo Zhang2,*  , Peng Shang1,* 
1College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, Xizang, People’s Republic of China
2National Engineering Laboratory for Animal Breeding/Beijing Key Laboratory for Animal Genetic Improvement, China Agricultural University, Beijing, People’s Republic of China
3College of Animal Sciences and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, People’s Republic of China
Correspondence:  Bo Zhang,Email: nemoshpmh@126.com
Peng Shang,Email: nemoshpmh@126.com
Received: 16 May 2020   • Revised: 23 June 2020   • Accepted: 16 May 2020
Abstract
Objective
Tibetan pigs, predominantly originating from the Tibetan Plateau, have been subjected to long-term natural selection in an extreme environment. To characterize the metabolic adaptations to hypoxic conditions, transcriptomic and proteomic expression patterns in the livers of Tibetan and Yorkshire pigs were compared.
Methods
RNA and protein were extracted from liver tissue of Tibetan and Yorkshire pigs (n = 3, each). Differentially expressed genes and proteins were subjected to Gene Ontology and Kyoto Encyclopedia of Genes and Genomes functional enrichment analyses.
Results
In the RNA-Seq and iTRAQ (Isobaric tags for relative and absolute quantitation) analyses, a total of 18,791 genes and 3390 proteins were detected and compared. Of these, 273 and 257 differentially expressed genes and proteins were identified. Evidence from functional enrichment analysis showed that many genes were involved in metabolic processes. The combined transcriptomic and proteomic analyses revealed that small molecular biosynthesis, metabolic processes, and organic hydroxyl compound metabolic processes were the major processes operating differently in the two breeds. The important genes include RDH16, APRT, PCYOX1, SORBS2, ENSSSCG00000036224, PLIN2, LAD1, KYAT1, and DDAH1.
Conclusion
The findings of this study provide novel insight into the high-altitude metabolic adaptation of Tibetan pigs.
Keywords: Hypoxia; Metabolic; Proteome; Tibetan pig; Transcriptome
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