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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0404    [Accepted] Published online October 13, 2020.
Integrated Transcriptomic Analysis on Small Yellow Follicles Reveals that Sosondowah Ankyrin Repeat Domain Family Member A Inhibits Chicken Follicle Selection
Conghao Zhong1  , Zemin Liu1  , Xibo Qiao2  , Li Kang1  , Yi Sun1  , Yunliang Jiang1,* 
1Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China
2Shandong Jihua Poultry Breeding Co. Ltd., Rizhao, China
Correspondence:  Yunliang Jiang, Tel: +86-538-8241593, Fax: +86-538-8241419, Email: yljiang723@aliyun.com
Received: 11 June 2020   • Revised: 19 July 2020   • Accepted: 2 September 2020
Abstract
Objective
Follicle selection is an important process in chicken egg laying. Among several small yellow (SY) follicles, the one exhibiting the highest expression of follicle stimulation hormone receptor (FSHR) will be selected to become a hierarchal follicle. The role of lncRNA, miRNA and other non-coding RNA in chicken follicle selection is unclear.
Methods
In this study, the whole transcriptome sequencing of SY follicles with different expression levels of FSHR in Jining Bairi hens was performed, and the expression of 30 randomly selected mRNAs, lncRNAs and miRNAs was validated by qPCR. Preliminary studies and bioinformatics analysis were performed on the selected mRNA, lncRNA, miRNA and their target genes. The effect of identified gene was examined in the granulosa cells of chicken follicles.
Results
Integrated transcriptomic analysis on chicken SY follicles differing in FSHR expression revealed 467 differentially expressed mRNA genes, 134 differentially expressed lncRNA genes and 34 differentially expressed miRNA genes, and sosondowah ankyrin repeat domain family member A (SOWAHA) was the common target gene of three miRNAs and one lncRNA. SOWAHA was mainly expressed in small white and SY follicles and was affected by FSH treatment in the granulosa cells. Knockdown of SOWAHA inhibited the expression of Wnt4 and StAR in the granulosa cells of prehierarchal follicles, while stimulated Wnt4 in hierarchal follicles. Overexpression of SOWAHA increased the expression of Wnt4 in the granulosa cells of prehierarchal follicles, decreased StAR and CYP11A1 in the granulosa cells of hierarchal follicles and inhibited the proliferation of granulosa cells.
Conclusion
Integrated analysis of chicken small yellow follicle transcriptomes identified SOWAHA as a network gene that is affected by FSH in granulosa cells of ovarian follicles. SOWAHA affected the expression of genes involved in chicken follicle selection and inhibited the proliferation of granulosa cells, suggesting an inhibitory role in chicken follicle selection.
Keywords: Chicken; Follicle Selection; Transcriptome; SOWAHA; Wnt4
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