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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0314    [Accepted] Published online August 30, 2020.
Correlation analysis of muscle amino acid deposition and gut microbiota profile of broilers reared at different ambient temperatures
Yuting Yang1  , Huan Gao1  , Xing Li1  , Zhenhui Cao1  , Meiquan Li2  , Jianping Liu3  , Yingying Qiao1  , Li Ma4  , Zhiyong Zhao5  , Hongbin Pan1,* 
1Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Science, Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201 China
2College of Agriculture, Kunming University, Kunming 650201, China
3Jiangsu Key Laboratory for Molecular and Medical Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
4Yunnan Vocational and Technical College of Agriculture, Kunming 650201, China
5Yunnan Animal Science and Veterinary Institute, Kunming 650201, China
Correspondence:  Hongbin Pan, Tel: +86-0871-65227798, Fax: +86-0871-65227798, Email: ynsdyz@163.com
Received: 8 May 2020   • Revised: 26 June 2020   • Accepted: 10 August 2020
Abstract
Objective
Temperature could influence protein and amino acid deposition as well as gut microbiota profile and composition. However, the specific effects of ambient temperature on amino acids deposition and gut microbiota composition remain insufficiently understood.
Methods
300 one-day-old Avian broilers were randomly divided into three groups and reared at high, medium and low temperature (HF, MF and LF), respectively. Breast muscle and fecal samples were collected for amino acid composition analysis and 16S rRNA gene sequence analysis.
Results
Our data showed that compared to the MF group, there was a decrease of muscle leucine and tyrosine (p<0.05), as well as an increase of methionine in the HF group (p<0.05) and a decrease of serine in the LF group. Examination of microbiota shift revealed that at genus level, the relative abundance of Turicibacter and Parabacteroides was increased in the HF group (p<0.05) and that the relative abundances of Pandoraea, Achromobacter, Prevotella, Brevundimonas and Stenotrophomonas in the LF group were higher than those in the MF group (p<0.05). In addition, there were substantial correlations between microbes and amino acids. In the HF group. Turicibacter was negatively correlated with aspartic acid and tyrosine, whereas Parabacteroides was positively correlated with methionine (p<0.05). In the LF group, there were multiple positive correlations between Achromobacter and arginine, isoleucine or tyrosine; between Prevotella and cysteine or phenylalanine; between Brevundimonas and cysteine; and between Stenotrophomonas and cysteine as well as a negative correlation between Stenotrophomonas and serine.
Conclusion
Our findings demonstrated that amino acid content of breast muscle and intestinal microbiota profile was affected by different ambient temperatures. Under heat exposure, augmented abundance of Parabacteroides was correlated with elevated methionine. Low temperature treatment may affect muscle tyrosine content through the regulation of Achromobacter.
Keywords: Broiler; Amino Acid Deposition; Fecal Microbiota Composition; Ambient Temperature


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