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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0118    [Accepted] Published online May 12, 2020.
Ram semen preserved at 0˚C with soybean lecithin Tris-based extender substituted for egg yolk
Jian-qing Zhao1,2  , Guo-liang Xiao3  , Wen-liang Zhu1,2  , Di Fang1,2  , Na Li1,2  , Chun-mei Han1,2  , Qing-hua Gao1,2,* 
1College of Life Sciences, Tarim University, Alar, Xinjiang 843300, China
2Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production &Construction Corps; Alar, Xinjiang 843300, China
3Kashgar Animal Husbandry Workstation, Kashi, Xinjiang 844000, China
Correspondence:  Qing-hua Gao, Tel: +86-13289976139, Fax: +86-0997-4681616, Email: gqhdky@126.com
Received: 22 February 2020   • Revised: 2 March 2020   • Accepted: 1 May 2020
The present study evaluated the preservation of ram semen at 0ºC using soybean lecithin with a Tris-fructose extender.
Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×108 sperm/mL, followed by cooling to 0ºC in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288h, respectively. Two hundred and twenty-three ewes were inseminated for 216h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination.
The results showed that there were no differences in sperm progressive motility at 0h, 24h, 72h and 144h (p>0.05). After 216h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05).
These results suggest that ram sperm is capable of fertilization after preservation at 0ºC with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.
Keywords: Ram; Semen; Diluent; Soy Lecithin; Egg Yolk
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