Go to Top Go to Bottom
Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0137    [Accepted] Published online May 12, 2020.
Effect of commercially-purified deoxynivalenol and zearalenone mycotoxins on microbial diversity of pig cecum contents
Kondreddy Eswar Reddy1  , Minji Kim1  , Ki Hyun Kim1  , Sang Yun Ji1  , Youlchang Baek1  , Ju Lan Chun1  , Hyun Jung Jung1,2  , Changyong Choe3  , Hyun Jeong Lee1,4  , Minseok Kim1,5,*  , Sung Dae Lee1,* 
1Animal Nutritional & Physiology Team, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea
2Department of Swine Science, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea
3Department of Animal Disease and Health, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Korea
4Department of Dairy Science, National Institute of Animal Science, Rural Development Administration, Cheonan 31000, Korea
5Department of Animal Science, College of Agriculture and Life Science, Chonnam National University, Gwangju 61186, Korea
Correspondence:  Minseok Kim,Email: mkim2276@@gmail.com
Sung Dae Lee,Email: leesd@korea.kr
Received: 3 March 2020   • Revised: 30 March 2020   • Accepted: 27 April 2020
Abstract
Objective
Deoxynivalenol (DON) and zearalenone (ZEN) are mycotoxins that frequently contaminate maize and grain cereals, imposing risks to the health of both humans and animals and leading to economic losses. The gut microbiome has been shown to help combat the effects of such toxins, with certain microorganisms reported to contribute significantly to the detoxification process.
Methods
We examined the cecum contents of three different dietary groups of pigs (control, as well as diets contaminated with 8 mg DON/kg feed or 0.8 mg ZEN/kg feed). Bacterial 16S rRNA gene amplicons were acquired from the cecum contents and evaluated by next-generation sequencing.
Results
A total of 2,539,288 sequences were generated with ~500 nucleotide read lengths. Firmicutes, Bacteroidetes, and Proteobacteria were the dominant phyla, occupying more than 96% of all three groups. Lactobacillus, Bacteroides, Megasphaera, and Campylobacter showed potential as biomarkers for each group. Particularly, Lactobacillus and Bacteroides were more abundant in the DON and ZEN groups than in the control. Additionally, 52,414 operational taxonomic units (OTUs) were detected in the three groups; those of Bacteroides, Lactobacillus, Campylobacter, and Prevotella were most dominant and significantly varied between groups. Hence, contamination of feed by DON and ZEN affected the cecum microbiota, while Lactobacillus and Bacteroides were highly abundant and positively influenced the host physiology.
Conclusion
Thus, Lactobacillus and Bacteroides play key roles in the process of detoxification and improving the immune response. We, therefore, believe that these results may be useful for determining whether disturbances in the intestinal microflora, such as the toxic effects of DON and ZEN, can be treated by modulating the intestinal bacterial flora.
Keywords: Mycotoxins; Pig; Intestine; Deoxynivalenol; Zearalenone; Detoxification


Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2020 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Close layer
prev next