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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.20.0060    [Accepted] Published online May 12, 2020.
Potential immune-modulatory effects of wheat phytase on the performance of a mouse macrophage cell line, Raw 264.7, exposed to long-chain inorganic polyphosphate
Jeongmin An1  , Jaiesoon Cho1,* 
Department of Animal Science and Technology, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea
Correspondence:  Jaiesoon Cho, Tel: +82-2-450-3375, Fax: +82-2-455-1044, Email: chojs70@konkuk.ac.kr
Received: 5 February 2020   • Revised: 3 April 2020   • Accepted: 27 April 2020
This experiment was conducted to find out the immunological effects of wheat phytase when long-chain inorganic polyphosphate (polyP) treated with wheat phytase was added to a macrophage cell line, Raw 264.7, when compared to intact long-chain polyP.
Nitric oxide (NO) production of Raw 264.7 cells exposed to P700, a long-chain polyP with an average of 1150 phosphate residues, treated with or without wheat phytase, was measured by Griess method. Phagocytosis assay of P700 treated with or without phytase in Raw 264.7 cells was investigated using neutral red uptake. The secretion of TNF-α by Raw 264.7 cells with wheat phytase-treated P700 compared to intact P700 was observed by using Mouse TNF-α ELISA kit.
P700 treated with wheat phytase effectively increased NO production of Raw 264.7 cells by 172 % when compared with intact P700 at 12 h exposure. At 5 mM of P700 concentration, wheat phytase promoted NO production of macrophages most strongly. P700, treated with wheat phytase, stimulated phagocytosis in macrophages at 12 h exposure by about 1.7-fold compared to intact P700. In addition, P700 treated with wheat phytase effectively increased in vitro phagocytic activity of Raw 264.7 cells at a concentration above 5 mM when compared to intact P700. P700 dephosphorylated by wheat phytase increased the release of TNF-α from Raw 264.7 cells by 143% over that from intact P700 after 6 h exposure. At the concentration of 50 μM P700, wheat phytase increased the secretion of cytokine, TNF-α, by 124% over that from intact P700.
In animal husbandry, wheat phytase can mitigate the long-chain polyP causing damage by improving the immune capabilities of macrophages in the host livestock. Thus, wheat phytase has inconceivable potential as an immunological modulator and future feed additive for regulating immune responses caused by inflammation induced by long-chain polyP from bacterial infection.
Keywords: Animal Husbandry; Immunological Effects; Long-Chain Inorganic Polyphosphate; Macrophage; Wheat Phytase

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