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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0418    [Accepted] Published online April 12, 2020.
Microsatellite based genetic characterization and bottleneck analysis of Kari and Madakhlasht sheep breeds from Chitral district of Khyber Pakhtunkhwa, Pakistan
Azmat Ullah1  , Sohail Ahmad2  , Muhammad Ibrahim2,* 
1Department of Animal Breeding and Genetics, Faculty of Animal Husbandry and Veterinary Sciences, The University of Agriculture Peshawar, Peshawar 25000, Pakistan
2Animal Biotechnology Division, Institute of Biotechnology and Genetic Engineering, The University of Agriculture Peshawar, Peshawar 25000, Pakistan
Correspondence:  Muhammad Ibrahim, Tel: +92-91-9221312, Fax: +92-91-9221262, Email: ibrahimfaqir@aup.edu.pk
Received: 22 May 2019   • Revised: 9 September 2019   • Accepted: 29 March 2020
Kari sheep show a large variation in body size and gestation length. In this study, we have analyzed the genetic differences between the three subtypes of Kari (based on body size) and another small size breed ‘Madakhlasht’ inhabiting the Chitral district of Pakistan.
Animals belonging to small Kari, medium Kari, large Kari and Madakhlasht sheep breeds were sampled from their breeding region and were characterized genotypically at the DNA level using microsatellite markers. A total of 120 blood samples (30 each) were collected from the four breed types. DNA from these samples were used to amplify 31 ovine specific microsatellite loci through PCR.
A total of 158 different alleles were detected across the 22 polymorphic loci with an average of 7.18 alleles per locus. Unique alleles were found in all four populations. Significant population differentiation was observed among all the populations. Madakhlasht sheep, phenotypically and geographically more distant from Kari, showed higher population differentiation and lower inbreeding and geneflow with Kari, compared to the values among Kari subtypes. Genetic distance revealed that Madakhlasht was the most distant population among the four populations, whereas, Small, Medium and Large subtypes of Kari sheep were relatively closer to each other. Medium and large Kari sheep were found in the state of mutation drift equilibrium, while small Kari and Madakhlasht showed the presence of bottleneck, indicating a reduction in their population sizes in the recent past.
The results suggest that the three subtypes of Kari possess unique genetic identity and are potentially important for further exploration of their specific characteristics such as productivity and reproduction, andexploitation of their unique values. Madakhlasht needs conservation on a priority basis for future breed improvement programs.
Keywords: Bottleneck Analysis; Genetic Diversity; Kari Sheep; Madakhlasht Sheep; Microsatellite Markers; Molecular Characterization
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