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Asian-Australas J Anim Sci > Volume 33(12); 2020 > Article
Animal Biotechnology
Asian-Australasian Journal of Animal Sciences 2020;33(12): 2008-2020.
DOI: https://doi.org/10.5713/ajas.19.0967    Published online March 12, 2020.
Dietary corn resistant starch regulates intestinal morphology and barrier functions by activating the Notch signaling pathway of broilers
Yingying Zhang1  , Yingsen Liu1  , Jiaolong Li1  , Tong Xing1  , Yun Jiang2  , Lin Zhang1,*  , Feng Gao1,* 
1College of Animal Science and Technology, Key Laboratory of Animal Origin Food Production and Safety Guarantee of Jiangsu Province, Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Joint International Research Laboratory of Animal Health and Food Safety, National Experimental Teaching Demonstration Center of Animal Science, Nanjing Agricultural University, Nanjing 210095, China
2School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210097, China
Correspondence:  Lin Zhang, Tel: +86-25-84399007, Fax: +86-25-84395314, Email: zhanglin2012@njau.edu.cn
Feng Gao, Tel: +86-25-84399007, Fax: +86-25-84395314, Email: gaofeng0629@sina.com
Received: 20 December 2019   • Revised: 24 January 2020   • Accepted: 21 February 2020
Abstract
Objective
This study was conducted to investigate the effects of dietary corn resistant starch (RS) on the intestinal morphology and barrier functions of broilers.
Methods
A total of 320 one-day-old broilers were randomly allocated to 5 dietary treatments: one normal corn–soybean (NC) diet, one corn–soybean–based diet supplementation with 20% corn starch (CS), and 3 corn–soybean–based diets supplementation with 4%, 8%, and 12% corn resistant starch (RS) (identified as 4% RS, 8% RS, and 12% RS, respectively). Each group had eight replicates with eight broilers per replicate. After 21 days feeding, one bird with a body weight (BW) close to the average BW of their replicate was selected and slaughtered. The samples of duodenum, jejunum, ileum, caecum digesta, and blood were collected.
Results
Birds fed 4% RS, 8% RS and 12% RS diets showed lower feed intake, BW gain, jejunal villus height (VH), duodenal crypt depth (CD), jejunal VH/CD ratio, duodenal goblet cell density as well as mucin1 mRNA expressions compared to the NC group, but showed higher concentrations of cecal acetic acid and butyric acid, percentage of jejunal proliferating cell nuclear antigen-positive cells and delta like canonical Notch ligand 4 (Dll4), and hes family bHLH transcription factor 1 mRNA expressions. However, there were no differences on the plasma diamine oxidase activity and D-lactic acid concentration among all groups.
Conclusion
These findings suggested that RS could suppress intestinal morphology and barrier functions by activating Notch pathway and inhibiting the development of goblet cells, resulting in decreased mucins and tight junction mRNA expression.
Keywords: Corn Resistant Starch; Intestinal Morphology; Barrier Function; Broiler
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