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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0541    [Accepted] Published online February 25, 2020.
Association of the thyroid hormone responsive spot 14 alpha gene with growth-related traits in Korean native chicken
Muhammad Cahyadi1  , Hee-Bok Park2  , Dong Won Seo3  , Shil Jin3  , Nuri Choi4  , Kang Nyeong Heo5  , Bo Seok Kang5  , Cheorun Jo6  , Jun Heon Lee3,* 
1Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta 57126, Indonesia
2Department of Animal Resources Science, Kongju National University, Yesan 32439, Korea
3Department of Animal and Dairy Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
4Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 54596, Korea
5Poultry Science Division, National Institute of Animal Science, RDA, Cheonan 331801, Korea
6Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Science, Seoul National University, Seoul 151921, Korea
Correspondence:  Jun Heon Lee, Tel: +82-42-821-5779, Fax: +82-42-825-9754, Email: junheon@cnu.ac.kr
Received: 3 July 2019   • Revised: 9 September 2019   • Accepted: 10 February 2020
Abstract
Objective
Thyroid hormone responsive spot 14 alpha (THRSP) has been used to investigate the regulation of de novo lipogenesis because the variation of THRSP mRNA content in the tissue affects directly the ability of that tissue to synthetize lipids. Also, this gene responds to thyroid hormone stimulation and high level of carbohydrate feeding or insulin-injection. This study was carried out to investigate variations within THRSP and their effects on body and carcass weights in Korean native chicken (KNC).
Methods
A total of 585 chickens which represent the five lines of KNC (Black, Gray-Brown, Red-Brown, White, and Yellow-Brown) were reared and body weight data were recorded every two weeks from hatch until 20 weeks of age. PCR-RFLP, DNA chips for Agilent 2100 Bioanalyzer, and Fluidigm Genotyping Technology, were applied to genotype selected markers. A linear mixed-effect model was used to access association between these SNP markers and growth-related traits.
Results
A total of 30 polymorphisms were investigated in THRSP. Of these, nine SNPs for loci were selected to perform association analyses. Significant associations were detected between g.-49G>T SNP with BW20, g.451T>C SNP with GR10-12 trait, and g.1432A>C SNP with GR14-16 trait and BW18. Moreover, diplotype of the THRSP gene significantly affected BW12 and GR10-12 traits. Diplotype of ht1/ht2 was favorable for BW12 and GR10-12 traits.
Conclusion
These results suggest that THRSP can be regarded as a candidate gene for growth traits in Korean native chicken.
Keywords: Body Weight; Growth; Korean Native Chicken; Association; THRSP


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