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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0785    [Accepted] Published online January 13, 2020.
Effect of γ-aminobutyric acid producing bacteria on in vitro rumen fermentation, growth performance, and meat quality of Hanwoo steer
Lovelia Leal Mamuad1  , Seon Ho Kim1  , Min Jung Ku2  , Sang Suk Lee1,* 
1Ruminant Nutrition and Anaerobe Laboratory, College of Bio-industry Science, Sunchon National University, Suncheon 57922, Korea
2Livestock Research Institute, Jeonnam Agricultural Research and Extension Services, Gangjin, Jeonnam 59213, Korea
Correspondence:  Sang Suk Lee, Tel: +82-61-750-3237, Fax: +82-61-750-3237, Email: rumen@sunchon.ac.kr
Received: 10 October 2019   • Revised: 3 December 2019   • Accepted: 17 December 2019
Abstract
Objective
The present study aimed to evaluate the effects of γ-aminobutyric acid (GABA)-producing bacteria (GPB) on in vitro rumen fermentation and on the growth performance and meat quality of Hanwoo steer.
Methods
The effects of GPB (Lactobacillus brevis YM 3-30)-produced and commercially available GABA were investigated using in vitro rumen fermentation. Using soybean meal as a substrate, either GPB-produced or commercially available GABA were added to the in vitro rumen fermentation bottles, as follows: no additive (Control), 2 g/L GPB (T1), 5 g/L GPB (T2), 2 g/L autoclaved GPB (T3), 5 g/L autoclaved GPB (T4), 2 g/L GABA (T5), and 5 g/L GABA (T6). In addition, 27 Hanwoo steers (602.06 ± 10.13 kg) were subjected to a 129-day feeding trial, during which they were fed daily with a commercially available total mixed ration that was supplemented with different amounts of GPB-produced GABA ((no additive (Control), 2 g/L GPB (T1), 5 g/L GPB (T2)). The degree of marbling was assessed using the nine-point beef marbling standard while endotoxin was analyzed using a Chromo-LAL (Limulus amebocyte lysate) test.
Results
In regards to in vitro rumen fermentation, the addition of GPB-produced GABA failed to significantly affect pH or total gas production but did increase the ammonia nitrogen (NH3-N) concentration (P<0.05) and reduce total biogenic amines (P<0.05). Animals fed the GPB-produced GABA diet exhibited significantly lower levels of blood endotoxins than control animals and yielded comparable average daily gain, feed conversion ratio, and beef marbling scores.
Conclusion
The addition of GPB improved in vitro fermentation by reducing biogenic amine production and by increasing both antioxidant activity and NH3-N production. Moreover, it also reduced the blood endotoxin levels of Hanwoo steers.
Keywords: Antioxidant; Biogenic Amines; γ-Aminobutyric Acid; Hanwoo Steers; In Vitro Rumen Fermentation


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