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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0745    [Accepted] Published online December 24, 2019.
Effects of gamma-aminobutyric acid and piperine on gene regulation in pig kidney epithelial cell lines
Juhyun Shin1  , Yoon-Mi Lee1  , Jeongheon Oh1  , Seunghwa Jung1  , Jae-Wook Oh1,* 
Department of Stem Cell and Regenerative Biotechnology, KIT, Konkuk University, Seoul, 05029 Korea
Correspondence:  Jae-Wook Oh, Tel: +82-2-2049-6271, Fax: +82-2-455-1044, Email: ohjw@konkuk.ac.kr
Received: 25 September 2019   • Revised: 1 November 2019   • Accepted: 25 September 2019
Abstract
Objective
Gamma-aminobutyric acid (GABA) and piperine (PIP) are both nutritional supplements with potential use in animal diets. The purpose of this study is to investigate the effect of GABA and/or PIP treatment on the gene expression pattern of a pig kidney epithelial cell line.
Methods
LLCPK1 cells were treated with GABA, PIP, or both, and then the gene expression pattern was analyzed using microarray. Gene ontology analysis was done using GeneOntology (Geneontology.org), and validation was performed using quantitative RT-PCR.
Results
Gene ontology enrichment analysis was used to identify key pathway(s) of genes whose expression levels were regulated by these treatments. Microarray results showed that GABA had a positive effect on the transcription of genes related to regulation of erythrocyte differentiation and that GABA and PIP in combination had a synergistic effect on genes related to immune systems and processes. Furthermore, we found that effects of GABA and/or PIP on these selected genes were controlled by JNK/p38 MAPK pathway. Conclusion: These results can improve our understanding of mechanisms involved in the effect of GABA and/or PIP treatment on pig kidney epithelial cells. They can also help us evaluate their potential as a clinical diagnosis and treatment.
Keywords: Gamma-aminobutyric Acid; Piperine; Pig Kidney Epithelial Cell; Microarray; Erythrocyte Differentiation; Immune System; MAPK Pathway


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