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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0624    [Accepted] Published online December 24, 2019.
Effects of season and single layer centrifugation on bull sperm quality in Thailand
Thanapol Nongbua1,2  , Apirak Utta3, Nutthee Am-in Am-in4  , Junpen Suwimonteerabutr4, Anders Johannisson1  , Jane Morrell1,* 
1Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, Uppsala 75007, Sweden
2Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham 44000, Thailand
3North-Eastern Bull Center, Bureau of Biotechnology in Livestock Production, DLD, Khon Kaen 40000, Thailand
4Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Correspondence:  Jane Morrell, Tel: +46-18671152, Fax: +46-18673545, Email: jane.morrell@slu.se
Received: 31 July 2019   • Revised: 4 September 2019   • Accepted: 28 October 2019
The aim of study was to investigate the effects of season and Single Layer Centrifugation (SLC) before cryopreservation on post-thaw bull sperm quality in Thailand.
Semen was collected from 6 bulls (Bos indicus) in summer, rainy season and winter 2014 – 2016. Semen characteristics, sperm morphology, sperm kinematics, viability, chromatin structure and mitochondrial membrane were evaluated. Meteorological data were available from the local meteorological station;
Season had an effect on semen characteristics in the raw ejaculate, with higher proportions of normal spermatozoa and lower abnormalities in winter than the other two seasons. Sperm kinematics, viability, DNA Fragmentation Index, and mitochondrial membrane potential were not different between seasons. Sperm samples selected by SLC had greater normal morphology and lower proportion with bent tails than controls and higher values of PRO, BCF, LIN, STR, WOB and lower values of SLOW, VAP, VCL and ALH than controls. In addition, SLC-selection had a favorable effect on PRO, VAP and WOB that differed among seasons.
Our results suggested that these bulls were well adapted to their location, with season having an effect on sperm morphology. Moreover, SLC could be used prior to cryopreservation, regardless of season, to enhance normal morphology and kinematics of bull sperm samples without adversely affecting other parameters of sperm quality. However, there was considerable variation among bulls in DNA fragmentation index, mitochondrial membrane potential and sperm viability. In addition, SLC had a positive effect on sperm morphology and sperm kinematics, which could be expected to influence fertility.
Keywords: Temperature; Humidity; Bovine Semen Quality; Colloid Centrifugation; Frozen Semen

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