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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0361    [Accepted] Published online December 24, 2019.
Antioxidant and antimicrobial activities of different enzymatic hydrolysates from desalted duck egg whit
Rommanee Thamamsena1, Deng Cheng Liu1,* 
Department of Animal Science, National Chung Hsing University, Taichung 40227, Taiwan
Correspondence:  Deng Cheng Liu, Tel: +886-4-22870613, Fax: +886-4-22860265, Email: dcliu@dragon.nchu.edu.tw
Received: 29 April 2019   • Revised: 11 September 2019   • Accepted: 12 November 2019
Abstract
Objective
The objective of this study was to look for optimal preparation of hydrolysates of desalted duck egg white powder (DDEWP) by the three different proteases and to investigate their antioxidant and antimicrobial properties.
Methods
DDEWP was hydrolyzed by three proteases, including pepsin (PEP), Bacillus spp. (BA) and natokinase (NAT) with three different enzyme concentrations (0.1, 0.3 and 0.5%), individually. The important key hydrolysis parameters such as hydrolysis degree, yield, antioxidant and antimicrobial activity were evaluated in this experiment.
Results
The results showed that the degree of hydrolysis (DH) of all treatments increased with increasing hydrolysis time and protease concentrations. The antioxidant and antimicrobial activities of the hydrolysates were affected by type and concentration of protease as well as hydrolysis time. Hydrolysis of PEP significantly (p < 0.05) obtained the highest yield of hydrolysates, however, both of BA and NAT showed substantially lower DH values and still did not exceed 5% by the end of hydrolysis. Among the different hydrolysates, PEP exhibited significantly higher DPPH radical scavenging activity than BA and NAT. All DDEWP hydrolysates from PEP had low ferrous ion chelating activity (<37%) that was significantly lower than that of NAT (>37-92%) and BA (30-79%). Besides, DDEWP hydrolysates of PEP presented significantly higher reducing power than BA and NAT. In antimicrobial activities, Escherichia coli, Salmonella typhimurium and Pseudomonas aeruginosa were not effectively inhibited by any DDEWP hydrolysates of PEP except for Staphylococcus aureus. Especially, the excellent antibacterial activity against S. aureus only was displayed in DDEWP hydrolysates of PEP 0.1%.
Conclusion
DDEWP hydrolysates from pepsin demonstrated significantly better DH, yield, DPPH radical scavenging activity and reducing power, furthermore, had excellent inhibitory on S. aureus due to large clear zone and moderated inhibitory in bactericidal inhibition.
Keywords: Desalted Duck Egg White; Hydrolysate; Antioxidant Activity; Antimicrobial Activity


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