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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0427    [Accepted] Published online November 12, 2019.
Effects of multi-strain probiotic supplementation on intestinal microbiota, tight junctions, and inflammation in young broiler chickens challenged with Salmonella enterica subsp. enterica
Chi Huan Chang1  , Po Yun Teng1  , Tzu Tai Lee1,2,*  , Bi Yu1,* 
1Department of Animal Science, National Chung Hsing University, 145 Xingda Rd., South Dist., Taichung City 402, Taiwan
2The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan
Correspondence:  Tzu Tai Lee,Email: ttlee@dragon.nchu.edu.tw
Bi Yu, Tel: +886-422840366, Fax: +886-422860265, Email: byu@dragon.nchu.edu.tw
Received: 24 May 2019   • Revised: 23 September 2019   • Accepted: 4 November 2019
Abstract
Objective
This study assessed the effects of probiotics on cecal microbiota, gene expression of intestinal tight junction proteins, and immune response in the cecal tonsil of broiler chickens challenged with Salmonella enterica subsp. enterica.
Methods
One-day-old broiler chickens (n = 240) were randomly allocated to four treatments: negative control (Cont), multi-strain probiotic-treated group (Pro), Salmonella-infected group (Sal), and multi-strain probiotic-treated and Salmonella-infected group (ProSal). All chickens except those in the Cont and Pro groups were gavaged with 1 × 108 cfu/mL of S. enterica subsp. enterica 4 days after hatching.
Results
Our results indicated that body weight, weight gain, and feed conversion ratio of birds were significantly reduced (p<0.05) by Salmonella challenge. Chickens challenged with Salmonella decreased cecal microbial diversity. Chickens in the Sal group exhibited abundant Proteobacteria than those in the Cont, Pro, and ProSal groups. Salmonella infection downregulated gene expression of Occludin, ZO1, and Mucin 2 in the jejunum and Occludin and Claudin in the ileum. Moreover, the Sal group increased gene expression of interferon-γ (IFN-γ), interleukin-6 (IL-6), IL-1β, and LPS-induced TNF-alpha factor (LITAF) and reduced levels of TGF-β4 and IL-10 compared with the other groups (p<0.05). However, chickens receiving probiotic diets increased Lactobacillaceae abundance and reduced Enterobacteriaceae abundance in the ceca. Moreover, supplementation with probiotics increased the mRNA expression of Occludin, ZO1, and Mucin 2 in the ileum (p<0.05). In addition, probiotic supplementation downregulated the mRNA levels of IFN-γ (p<0.05) and LITAF (p=0.075) and upregulated IL-10 (p=0.084) expression in the cecal tonsil.
Conclusion
The administration of multi-strain probiotics modulated intestinal microbiota, gene expression of tight junction proteins, and immunomodulatory activity in broiler chickens.
Keywords: Multi-strain Probiotics; Intestinal Microbiota; Tight Junctions; Inflammation; Salmonella enterica subsp. enterica


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