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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0438    [Accepted] Published online November 12, 2019.
miR-140 inhibits porcine fetal fibroblasts proliferation by directly targeting IGF1R and indirectly inhibiting IGF1R expression via SOX4
Hongwei Geng1  , Linlin Hao1  , Yunyun Cheng1  , Chunli Wang1  , Wenzhen Wei1  , Rui Yang1  , Haoyang Li1  , Ying Zhang1,*  , Songcai Liu1,2,* 
1College of Animal Science, Jilin University, 5333 Xi’an Road, Changchun, Jilin 130062, China
2Five-Star Animal Health Pharmaceutical Factory of Jilin Province, Changchun, Jilin 130062, China
Correspondence:  Ying Zhang,Email: z_ying@jlu.edu.cn
Songcai Liu, Tel: +86-18843181673, Fax: +86-89424385, Email: songcai@jlu.edu.cn
Received: 31 May 2019   • Revised: 11 July 2019   • Accepted: 28 October 2019
Abstract
Objective
This study aimed to elucidate the effect of miR-140 on the proliferation of porcine fetal fibroblasts (PFFs) and identify the target genes of miR-140 in PFFs.
Methods
In this study, bioinformatics software was used to predict and verify miR-140 target genes. Quantitative polymerase chain reaction and western blot were used to detect the relationship between miR-140 and its target genes in PFFs. Dual luciferase reporter gene assays were performed to assess the interactions among miR-140, type 1 insulin-like growth factor receptor (IGF1R), and SRY-box 4 (SOX4). The effect of miR-140 on the proliferation of PFFs was measured by CCK-8 when PFFs were transfected with a miR-140 mimics or inhibitor. The transcription factor SOX4 binding to promoter of IGF1R was detected by dual luciferase reporter gene assays and chromatin immunoprecipitation assay (ChIP).
Results
miR-140 directly targeted IGF1R to inhibit proliferation of porcine fetal fibroblasts (PFFs). Meanwhile, miR-140 targeted transcription factor SOX4 that bound to promoter of porcine IGF1R to indirectly inhibit the expression of IGF1R. In addition, miR-140 inhibitor promoted PFFs proliferation, which is abrogated by SOX4 or IGF1R knockdown.
Conclusion
miR-140 inhibits porcine fetal fibroblasts proliferation by directly targeting IGF1R and indirectly inhibiting IGF1R expression via SOX4.
Keywords: miR-140; IGF1R; SOX4; Proliferation
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