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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0533    [Accepted] Published online October 22, 2019.
Characterization of long non-coding RNA according to sex in Hanwoo cattle
Jae-Young Choi1  , Donghyun Shin2  , Jae-Don Oh2,* 
1Subtropical Livestock Research Institute, National Institute of Animal Science, RDA, Jeju 63242, Korea
2Department of Animal Biotechnology, College of Agricultural and Life Sciences, Chonbuk National University, Jeonju 54896, Korea
Correspondence:  Jae-Don Oh, Tel: +82-01041725001, Fax: +82-63-219-5505, Email: oh5ow@naver.com
Received: 16 July 2018   • Revised: 19 September 2018   • Accepted: 14 October 2019
Abstract
Objective
Cattle were some of the first animals domesticated by humans for the production of milk, meat, etc. Long non-coding RNAs (lncRNAs) are defined as molecules longer than 200 bp in non-protein coding transcripts. lncRNAs are known to function in regulating gene expression and are currently being studied in a variety of livestock, including cattle. The purpose of this study is to analyze the characteristics of lncRNA according to sex in Hanwoo cattle.
Methods
This study was conducted using the skeletal muscles of nine Hanwoo cattle, including bulls, steers, and cows. RNA was extracted from the skeletal muscles of the animals. Sequencing was conducted using Illumina HiSeq2000 and mapped to the bovine taurus genome. The expression levels of lncRNAs were measured by DEGseq and the QTL database was used to identify QTLs associated with lncRNA. A python script was used to match the nearby genes.
Results
In this study, the transcript expression patterns of bulls, steers, and cows were identified. We also identified significantly differentially expressed lncRNAs in bulls, steers, and cows. In addition, characteristics of lncRNA that were expressed differentially in the muscles according to the sex of the Hanwoo cattle were identified. As a result, we found that lncRNAs that were differentially expressed according to sex were related to shear force and body weight.
Conclusion
This study classified and characterized lncRNA that were differentially expressed according to sex in Hanwoo cattle. We believe that the characterization of these lncRNA will be helpful for future studies of the physiological mechanisms of Hanwoo cattle.
Keywords: Hanwoo; Sex; LncRNA; QTL; Muscle


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