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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0344    [Accepted] Published online October 21, 2019.
A lower cost method of preparing corn stover for Irpex lacteus treatment by ensiling with lactic acid bacteria
Sasa Zuo1  , Di Jiang1  , Dongze Niu1  , Mingli Zheng1  , Ya Tao1  , Chuncheng Xu1,* 
College of Engineering, China Agricultural University, Beijing 100083, China
Correspondence:  Chuncheng Xu, Tel: +86-010-62736480, Fax: +86-010-62736480, Email: xucc@cau.edu.cn
Received: 24 April 2019   • Revised: 12 July 2019   • Accepted: 18 September 2019
Abstract
Objective
This study investigated a method of preparing corn stover for Irpex lacteus treatment to improve its in vitro rumen degradability under non-sterile conditions.
Methods
Corn stover was inoculated with Lactobacillus plantarum, Lactobacillus buchneri, and an equal mixture of these strains, and the stover was ensiled for 0, 3, 7, 14 and 28 days. After each period of time, a portion of the silage was sampled, to assess the silage quality, and another portion of the silage was further treated with I. lacteus at 28 °C for 28 d. All of the samples were analyzed for fermentation quality, chemical composition, and in vitro gas production (IVGP) as a measure of rumen fermentation capacity.
Results
Lactic acid bacteria (LAB) was found to improve the silage quality of the corn stover, and the corn stover silage inoculated with L. plantarum produced more lactic acid and higher IVGP than other silage groups. The I. lacteus colonies flourished on in the early stage of corn stover silage, especially on the 3-d corn stover silage inoculated with both L. plantarum and L. buchneri. This led to an 18% decrease in the acid detergent lignin (ADL) content, and a 49.6% increase in IVGP compared with the raw stover.
Conclusion
The combination of ensiling with the mixed LAB inoculation and I. lacteus treatment provided a cost-effective method for the improvement of the IVGP of corn stover from 164.8 ml/g OM to 246.6 ml/g OM.
Keywords: Ensiling; Irpex lacteus; Corn Stover; Sterilization; In Vitro Gas Production
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