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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0393    [Accepted] Published online August 26, 2019.
Effects of dietary Antrodia cinnamomea fermented product supplementation on metabolism pathways of antioxidant, inflammatory, and lipid metabolism pathways-a potential crosstalk
M. T. Lee1  , W. C. Lin1  , L. J. Lin2  , S. Y. Wang3  , S. C. Chang4  , Tzu-Tai Lee1,5,* 
1Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan
2Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, 404, Taiwan
3Department of Forestry, National Chung Hsing University, Taichung, 402, Taiwan
4Kaohsiung Animal Propagation Station, Livestock Research Institute, Council of Agriculture, 912, Taiwan
5The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, 402, Taiwan
Correspondence:  Tzu-Tai Lee, Tel: +886-4-22840366, Fax: +886-4-2286-0265, Email: ttlee@dragon.nchu.edu.tw
Received: 11 May 2019   • Revised: 17 June 2019   • Accepted: 19 August 2019
Abstract
Objective
This study was conducted to fathom the underlying mechanisms of nutrition intervention and redox sensitive transcription factors regulated by Antrodia cinnamomea fermented product (FAC) dietary supplementation in broiler chickens.
Methods
Four hundreds d-old broilers (41±0.5 g/bird) assigned to 5 groups were examined after consuming control diet, or control diet replaced with 5% wheat bran (WB), 10% WB, 5% FAC and 10% FAC. Liver mRNA expression of antioxidant, inflammatory and lipid metabolism pathways were analyzed. Prostaglandin E2 (PGE2) concentration in each groups were tested in the chicken peripheral blood mononuclear cells (cPBMCs) of 35-d old broilers to represent the stress level of the chickens. Furthermore, these cells were stimulated with 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH) and lipopolysaccharide (LPS) to evaluate the cell stress tolerance by measuring cell viability and oxidative species.
Results
HO-1, GST, GCLC, and SOD, and Nrf2 that regulates the above antioxidant genes were all up-regulated significantly in FAC groups. ROMO1 and NOX1 were both rather down-regulated in 10% FAC group as comparison with two WB groups. Despite expressing higher level than control group, birds receiving diet containing FAC had significantly lower expression level in NF-κB and other genes (iNOS, TNF-α, IL-1β, NLRP3 and COX2) involving in inflammatory pathways. Additionally, except for HMGCoAR that showed relatively higher in both groups with WB, LPL, ACC, FAS, FABP, FADS2 and PPAR-α genes were expressed in higher level in 10% FAC group. In support of above results, promoted Nrf2 and inhibited NF-κB nuclear translocation in chicken liver were found in FAC containing groups. H2O2 and NO levels induced by LPS and AAPH in cPBMCs were compromised in FAC containing diet. In 35-d-old birds, PGE2 production in cPBMCs was also suppressed while offering FAC in diet.
Conclusion
The FAC may promote Nrf2 antioxidant pathway and positively regulate lipid metabolism, both are potential inhibitor of NF-κB inflammatory pathway.
Keywords: Antrodia cinnamomea; Boiler Chickens; Nrf2; NF-κB; Lipid Metabolism


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