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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0080    [Accepted] Published online August 23, 2019.
Conjugated linoleic acid producing potential of lactobacilli isolated from goat (AXB) rumen fluid samples
Amrish Kumar Tyagi1  , Sachin Kumar1,2,*  , Prasanta Kumar Choudhury1,3  , Bhawna Tyagi1  , Nitin Tyagi1 
1Rumen Biotechnology Lab, Animal Nutrition Division, ICAR-National Dairy Research Institute, Karnal, Haryana, 132001, India
2Gut Microbial Function, CSIRO Agriculture and Food, Queensland Bioscience Precinct, St Lucia, Brisbane, 4067, Australia
3Dairy Technology Department, SoABE, Centurion University of Technology and Management, Paralakhemundi-761211, Odisha, India
Correspondence:  Sachin Kumar, Tel: 0184-2259069, Fax: 0184-2250042, Email: arensachin@gmail.com
Received: 23 January 2019   • Revised: 24 May 2019   • Accepted: 26 July 2019
Abstract
Objective
The present investigation was aimed to explore the potential of lactobacilli for conjugated linoleic acid (CLA) production, isolated from rumen fluid samples of lactating goats.
Methods
A total of 64 isolates of lactobacilli were isolated using MRS agar from rumen fluid of goats and further subjected to morphological and biochemical characterizations. Isolates found as Gram-positive, catalase negative rods were presumptively identified as Lactobacillus species and further confirmed by genus specific PCR. The phylogenetic tree was constructed from the nucleotide sequences using MEGA6.
Results
Out of the 64 isolates, 23 isolates were observed positive for CLA production by linoleate isomerase (LAI) gene-based amplification and quantitatively by UV-spectrophotometric assay for the conversion of linoleic acid (LA) to CLA as well as gas chromatography-based assay. In all Lactobacillus species cis9, trans11 isomer was observed as the most predominant CLA isomer. These positive isolates were identified by 16S rRNA gene-based PCR sequencing and identified to be different species of L. ingluviei (2), L. salivarius (2), L. curvatus (15) and L. sakei (4).
Conclusion
The findings of the present study concluded that lactic acid bacteria (LAB) isolated from ruminal fluid samples of goat have the potential to produce bioactive CLA and may be applied as direct fed microbial to enhance the nutraceutical value of animal food products.
Keywords: Biohydrogenation; Conjugated Linoleic Acid; Goat; Lactobacilli; Rumen


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