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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0313    [Accepted] Published online August 3, 2019.
Temporal expression profiling of long noncoding RNA and mRNA in the peripheral blood during porcine development
Yiren Gu2  , Rui Zhou1  , Long Jin1  , Xuan Tao2  , Zhijun Zhong2  , Xuemei Yang2  , Yan Liang2  , Yuekui Yang2  , Yan Wang2  , Xiaohui Chen2  , Jianjun Gong2  , Zhiping He2  , Mingzhou Li1,*  , Xuebin Lv2,* 
1Animal Breeding and Genetics Key Laboratory of Sichuan Province, Sichuan Animal Science Academy, Chengdu, Sichuan 610066, China
2Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China
Correspondence:  Mingzhou Li, Tel: +86-13348870312, Fax: +86-028-86290987, Email: mingzhou.li@sicau.edu.cn
Xuebin Lv,Email: lvxuebinsasa@163.com
Received: 12 April 2019   • Revised: 29 May 2019   • Accepted: 23 July 2019
Abstract
Objective
We investigated the temporal expression profiles of long noncoding RNA (lncRNA) and mRNA in the peripheral blood of pigs during development and identified the lncRNAs that are related to the blood-based immune system.
Methods
Peripheral blood samples were obtained from the pigs at 0, 7, 28 and 180 days and 2 years of age. RNA sequencing was performed to survey the lncRNA and mRNA transcriptomes in the samples. Short time-series expression miner (STEM) was used to show temporal expression patterns in the mRNAs and lncRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to assess the genes’ biological relevance. To predict the functions of the identified lncRNAs, we extracted mRNAs that were nearby loci and highly correlated with the lncRNAs.
Results
In total of 5,946 lncRNA and 12,354 mRNA transcripts were identified among the samples. STEM showed that most lncRNAs and mRNAs had similar temporal expression patterns during development, indicating the expressional correlation and functional relatedness between them. The five stages were divided into two classes: the suckling period and the late developmental stage. Most genes were expressed at low level during the suckling period, but at higher level during the late stages. Expression of several T-cell-related genes increased continuously during the suckling period, indicating that these genes are crucial for establishing the adaptive immune system in piglets at this stage. Notably, lncRNA TCONS-00086451 may promote blood-based immune system development by upregulating nuclear factor of activated T-cells cytoplasmic 2 (NFATC2) expression.
Conclusion
This study provides a catalog of porcine peripheral blood-related lncRNAs and mRNAs and reveals the characteristics and temporal expression profiles of these lncRNAs and mRNAs during peripheral blood development from the newborn to adult stages in pigs.
Keywords: Long Noncoding RNA; mRNA; Pig; Peripheral Blood; Immune System Development


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