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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0260    [Accepted] Published online August 3, 2019.
Comparison for immunophysiological responses of Jeju and Thoroughbred horses after exercise
Saichit Khummuang1  , Hyo Gun Lee1  , Sang Seok Joo1  , Jeong-Woong Park1  , Jae-Young Choi1  , Jin Hyeog Oh1  , Kyoung Hwan Kim1  , Hyun-Hee Youn1  , Myunghoo Kim1,*  , Byung-wook Cho1,* 
Department of Animal Science, College of Natural Resources and Life Sciences, Pusan National University, Miryang 50463, Korea
Correspondence:  Myunghoo Kim,Email: mhkim18@pusan.ac.kr
Byung-wook Cho, Tel: +82-1092079392, Fax: +82-553505519, Email: bwcho@pusan.ac.kr
Received: 28 March 2019   • Revised: 3 June 2019   • Accepted: 12 July 2019
Abstract
Objective
The study was conducted to investigate variations in the immunophysiological responses to exercise-induced stress in Jeju and Thoroughbred horses.
Methods
Blood samples were collected from the jugular veins of adult Jeju (N = 5) and Thoroughbred (N = 5) horses before and after 30 min of exercise. The hematological, biochemical, and immunological profiles of the blood samples were analyzed. The samples were prepared blood smears then were stained and observed under a microscope. The concentration of cell-free (cf) DNA in the plasma was determined using real time PCR. Peripheral blood mononuclear cells (PBMCs) and polymorphonuclear cells (PMNs) were separated using PolymorphprepTM, and the expression of various stress-related and chemokine receptor genes was measured using reverse transcriptase (RT) and real-time PCR.
Results
After exercise, Jeju and Thoroughbred horses displayed stress responses with significantly increased rectal temperatures, cortisol levels, and muscle catabolism-associated metabolites. Red blood cell indices were significantly higher in Thoroughbred horses than in Jeju horses after exercise. In addition, exercise-induced stress triggered the formation of neutrophil extracellular traps (NETs) and reduced platelet counts in Jeju horses but not in Thoroughbred horses. HSP72 and HSPA6 expression is rapidly modulated in response to exercise-induced stress in the PBMCs of Jeju horses. The expression of CXCR4 in PBMCs was higher in Thoroughbred horses than in Jeju horses after exercise.
Conclusion
In summary, the different immunophysiological responses of Jeju and Thoroughbred horses explain the differences in the physiological and anatomical properties of the two breeds. The physiology of Thoroughbred horses makes them suitable for racing as they are less sensitive to exercise-induced stress compared to that of Jeju horses. This study provides a basis for investigating the link between exercise-induced stresses and the physiological alteration of horses. Hence, our findings show that some of assessed parameters could be used to determine the endurance performance of horses.
Keywords: Jeju Horse; Thoroughbred Horse; Exercise; Blood Analysis; Immunological Gene Expression


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