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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.19.0092    [Accepted] Published online May 28, 2019.
Impact of Ecklonia stolonifera extract on in vitro ruminal fermentation characteristics, methanogenesis, and microbial populations
Shin Ja Lee1  , Jin Suk Jeong2  , Nyeon Hak Shin3  , Su Kyoung Lee4  , Hyun Sang Kim5  , Jun Sik Eom5  , Sung Sill Lee1,2,* 
1Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University, Jinju 52828, Korea
2Division of Applied Life Science (BK21Plus) and Institute of Agriculture & Life Science (IALS), Gyeongsang National University, Jinju 52828, Korea
3Livestock Experiment Station, Gyeongsangnamdo Livestock Promotion Research Institute, Sancheong 52733, Korea
4Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Korea
5Division of Applied Life Science (BK21Plus), Gyeongsang National University, Jinju 52828, Korea
Correspondence:  Sung Sill Lee, Tel: +82-55-772-1883, Fax: +82-55-772-1889, Email: lss@gnu.ac.kr
Received: 1 February 2019   • Revised: 8 April 2019   • Accepted: 16 May 2019
Abstract
Objective: This study was conducted to evaluate the effects of E. stolonifera extract addition on in vitro ruminal fermentation characteristics, methanogenesis and microbial populations.

Methods

Cannulated Holstein cows (450 ± 30 kg) consuming timothy hay and a commercial concentrate (60:40, w/w) twice daily (09:00 and 17:00) at 2% of body weight with free access to water and mineral block were used as rumen fluid donors. In vitro fermentation experiment, with timothy hay as substrate, was conducted for up to 72 h, with E. stolonifera extract added to achieve final concentration 1, 3 and 5% on timothy hay basis.

Results

Administration of E. stolonifera extract to a ruminant fluid-artificial saliva mixture in vitro increased the total gas production. Unexpectedly, E. stolonifera extracts appeared to increase both methane emissions and hydrogen production, which contrasts with previous observations with brown algae extracts used under in vitro fermentation conditions. Interestingly, real-time polymerase chain reaction indicated that as compared with the untreated control the ciliate-associated methanogen and Fibrobacter succinogenes populations decreased, whereas the Ruminococcus flavefaciens population increased as a result of E. stolonifera extract supplementation.

Conclusions

E. stolonifera showed no detrimental effect on rumen fermentation characteristics and microbial population. Through these results E. stolonifera has potential as a viable feed supplement to ruminants.
Keywords: Ecklonia stolonifera Extract; In vitro Fermentation; Methane Emission; Microbial Population


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