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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0512    [Accepted] Published online February 14, 2019.
Effects of feed intake on the diversity and population density of homoacetogens in the large intestine of pigs
Hiroki Matsui1,*  , Ayumi Mimura1, Sakiko Maekawa1, Tomomi Ban-Tokuda1
Graduate School of Bioagricultural Sciences, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan
Correspondence:  Hiroki Matsui, Tel: +81-59-231-9593, Fax: +81-59-231-9540, Email: matsui@bio.mie-u.ac.jp
Received: 5 July 2018   • Revised: 30 August 2018   • Accepted: 12 February 2019
Abstract
Objective: Homoacetogens play important roles in the production of acetate in the large intestine of monogastric mammals. However, their diversity in the porcine large intestine is still unknown. Marker gene analysis was performed to assess the effects of energy level on the diversity and population densities of homoacetogens in porcine feces.

Methods

Crossbred pigs were fed high or low energy-level diets. The high-intake (HI) diet was sufficient to allow a daily gain of 1.2 kg. The low-intake (LI) diet provided 0.6 times the amount of energy as the HI diet. Genetic diversity was analyzed using formyltetrahydrofolate synthetase (FTHFS) gene (fhs) clone libraries derived from fecal DNA samples. fhs DNA copy numbers were quantified using real-time PCR.

Results

A wide variety of fhs sequences was recovered from animals in both treatments. No differences in fhs clone libraries between the HI and LI groups were found. During the experimental period, no significant differences in the proportion of fhs copy numbers were observed between the two treatment groups.
Conclusion
This is the first reported molecular diversity analysis using specific homoacetogen marker genes from the large intestines of pigs. There was no observable effect of feed intake on acetogen diversity.
Keywords: Feed Intake; Formyltetrahydrofolate Synthetase (FTHFS) Gene (fhs); Homoacetogen; Large Intestine; Pig


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