• Home
  • E-Submission
  • Sitemap
  • Contact Us
Asian-Australas J Anim Sci. Search


Go to Top Go to Bottom
Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0671    [Accepted] Published online January 4, 2019.
Assessment of Mulberry leaf as a potential feed supplement for animal feeding in P.R. China
Ming Cai1,2  , Lan Mu1,2  , Zong li Wang1,4, Jian yong Liu2, Tian liang Liu2, Metha Wanapat3,*  , Bi zhi Huang2,*
1State Key Laboratory of Grassland Agro-ecosystems, College of Pastoral Agricultural Science and Technology, Lanzhou University, Lanzhou 730020, Gansu, China
2Yunnan Academy of Grassland and Animal Science, Kunming 650212, Yunnan, China
3Tropical Feed Resources Research and Development Center, Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
4China Animal Health And Epidemiology Center, Qingdao 266032, Shandong, China
Correspondence:  Metha Wanapat,Email: metha@kku.ac.th
Bi zhi Huang, Tel: +86-13888053808, Fax: +86-0871-67391020, Email: hbz@ynbp.cn
Received: 6 September 2018   • Revised: 19 November 2018   • Accepted: 14 December 2018
Objective: Mulberry (Morus alba L.) is a cultivated shrub grown widely in the sub-tropical and tropical areas. It has been shown that mulberry leaf contains high levels of protein while having polyphenols as phytonutrients. Therefore, it is important to conduct an experiment to assess potential toxic level from mulberry on behavior, blood hematological and coagulation parameter using Sprague Dawley rats.


Both male and female Sprague-Dawley rats (SD rats) were given an intragastric administration of respective treatments of mulberry leaf intakes (control, low and high levels). Parameters of feed intake, hematological and coagulation of blood parameters, as well as liveweight changes were taken during the 7d of adaptation, 28d of treatment exposure and 14d of recovery periods, respectively. All treatment data were statistically analyzed using ANOVA of SPSS17.0 for Windows Statistical Software following the Randomized complete block design with sex as a block.


Most of the parameters of the physical symptoms of the SD rats, were not significantly different (p>0.05) when compared with that of the control group. Those which remain unchanged in each dose group were, body weight gain, feed intake, the hematology and coagulation indexes. Although, there were a few individual indicators that were abnormal, but the overall physiological appearance of the rats were normal.

Results under this experiment revealed normal hematological and coagulation parameters of the SD rats in both male and female, although the weight gain of female rats was significantly affected more than those of the male rats. Under this study,the use of mulberry leaf up to 2g/kg·BW did not result in abnormal phenomenon in the SD rats. These findings would offer useful information for further in vivo feeding trials in animals to extensively use of mulberry leaf to improve animal production, particularly in P.R. China.
Keywords: Mulberry Leaf; SD Rat; Toxic Level; Protein Resource

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2019 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next