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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0739    [Accepted] Published online January 2, 2019.
Effect of oocyte chromatin status in porcine follicles on the embryo development in vitro
Joo Bin Lee1, Min Gu Lee1, Tao Lin1, Hyeon Yeong Shin1, Jae Eun Lee1, Jung Won Kang1, Dong-Il Jin1,*
Division of Animal & Dairy Science, Chungnam National University Daejeon 34134, Korea
Correspondence:  Dong-Il Jin, Tel: +82-42-821-7854, Fax: +82-42-821-5771, Email: dijin@cnu.ac.kr
Received: 5 October 2018   • Revised: 13 November 2018   • Accepted: 4 December 2018
Abstract
Objective: The main goal of this study was to provide a morphological indicator that could be used to select high-quality oocytes of appropriate meiotic and developmental capabilities in pig. The higher quality of immature oocytes, the higher success rates of IVM and IVF. Thus, prior to the IVM culture, it is important to characterize oocytes morphologically and biochemically in order to assess their quality. Two of the largest indicators of oocyte quality are the presence of cumulus cells and status of chromatin. To investigate the effects of porcine oocyte chromatin configurations on the developmental capacity of blastocysts, we assessed oocyte chromatin status according to follicle size and measured the developmental potency of blastocysts.

Methods

To sort by follicle size, we divided the oocytes into three groups (less than 1 mm, 1-3 mm and more than 3 mm in diameter). To assess chromatin configuration, the oocytes were assessed for their stages (SN GV, NSN GV, GVBD, MI, proMII and MII) at different maturation times (22, 44 and 66 h). To assess the development rate, oocytes of each follicle size were subjected to parthenogenetic activation for further development. Finally, GV oocytes were grouped by their chromatin configuration (SN, SN/NSN and NSN) and their global transcriptional levels were measured.

Results

SN GV oocytes were more suitable for IVF than NSN GV oocytes. Moreover, oocytes collected from the larger follicles had a greater distribution of SN GV oocytes and a higher developmental capacity during IVM, reaching MII more quickly and developing more often to blastocysts.
Conclusion
Porcine oocytes with high-level meiotic and developmental capacity were identified by analyzing the relationship between follicle size and chromatin configuration. The porcine oocytes from large follicles had a significantly higher SN status in which the transcription level was low, could be better in the degree of meiotic progression and developmental capacity.
Keywords: Porcine Oocyte Chromatin, Follicle Size, Oocyte Maturation, Embryo Development


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