• Home
  • E-Submission
  • Sitemap
  • Contact Us
Asian-Australas J Anim Sci. Search

CLOSE


Go to Top Go to Bottom
Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0302    [Accepted] Published online May 31, 2018.
Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts
Jeong-Woong Park1, Jeong Hyo Lee1, Seo Woo Kim1, Ji Seon Han1, Kyung Soo Kang2, Sung-Jo Kim3,*, Tae Sub Park1,*
1Seoul National University, Pyeongchang-gun, Gangwon-do, Korea
2Bio Division, Medikinetics, Inc. , Pyeongtaek-si, Korea
3Division of Cosmetics and Biotechnology, Hoseo University, Asan-si, Korea
Correspondence:  Sung-Jo Kim,Email: sungjo@hoseo.edu
Tae Sub Park, Tel: +82-33-339-5721, Fax: +82-33-339-5763, Email: taesubpark@snu.ac.kr
Received: 16 April 2018   • Revised: 9 May 2018   • Accepted: 29 May 2018
Abstract
Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis.

Methods

We conducted mRNA next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative RT-PCR.

Results

Through RNA sequencing analysis, we found that the transcription levels of six genes (TNNC1, MYL1, MYL3, PLN, CAV3, and CASQ2) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts cells. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle.
Conclusion
These results indicated that calcium regulation might plays a critical role on muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.
Keywords: Quail; Muscle differentiation; Calcium; RNA-sequencing


ABOUT
SPECIALTIES
BROWSE ARTICLES
FOR AUTHORS AND REVIEWERS
Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2018 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next