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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0302    [Accepted] Published online May 31, 2018.
Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts
Jeong-Woong Park1, Jeong Hyo Lee1, Seo Woo Kim1, Ji Seon Han1, Kyung Soo Kang2, Sung-Jo Kim3,*, Tae Sub Park1,*
1Seoul National University, Pyeongchang-gun, Gangwon-do, Korea
2Bio Division, Medikinetics, Inc. , Pyeongtaek-si, Korea
3Division of Cosmetics and Biotechnology, Hoseo University, Asan-si, Korea
Correspondence:  Sung-Jo Kim,Email: sungjo@hoseo.edu
Tae Sub Park, Tel: +82-33-339-5721, Fax: +82-33-339-5763, Email: taesubpark@snu.ac.kr
Received: 16 April 2018   • Revised: 9 May 2018   • Accepted: 29 May 2018
Objective: In the poultry industry, the most important economic traits are meat quality and carcass yield. Thus, many studies were conducted to investigate the regulatory pathways during muscle differentiation. To gain insight of muscle differentiation mechanism during growth period, we identified and validated calcium-related genes which were highly expressed during muscle differentiation through mRNA sequencing analysis.


We conducted mRNA next-generation-sequencing (NGS) analysis of mRNA from undifferentiated QM7 cells and differentiated QM7 cells (day 1 to day 3 of differentiation periods). Subsequently, we obtained calcium related genes related to muscle differentiation process and examined the expression patterns by quantitative RT-PCR.


Through RNA sequencing analysis, we found that the transcription levels of six genes (TNNC1, MYL1, MYL3, PLN, CAV3, and CASQ2) particularly related to calcium regulation were gradually increased according to days of myotube differentiation. Subsequently, we validated the expression patterns of calcium-related genes in quail myoblasts cells. These results indicated that TNNC1, MYL1, MYL3, PLN, CAV3, CASQ2 responded to differentiation and growth performance in quail muscle.
These results indicated that calcium regulation might plays a critical role on muscle differentiation. Thus, these findings suggest that further studies would be warranted to investigate the role of calcium ion in muscle differentiation and could provide a useful biomarker for muscle differentiation and growth.
Keywords: Quail; Muscle differentiation; Calcium; RNA-sequencing

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