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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.17.0911    [Accepted] Published online April 25, 2018.
Novel splice isoforms of pig MYNN and their diverse mRNA expression patterns
Meng Li1, Xiaohong Guo1, Pengfei Gao1, Guoqing Cao1, Zhimin Cheng1, Wanfeng Zhang1, Jianfeng Liu2, Xiaojun Liu3, Bugao Li1,*
1College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801, China
2Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing , China
3College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, Henan, China
Correspondence:  Bugao Li, Tel: +86-138-3540-1655, Fax: +86-0354-628-8228, Email: 13994576150@163.com
Received: 17 December 2017   • Revised: 21 February 2018   • Accepted: 23 April 2018
Abstract
Objective: The aim of this study was to clone alternative splicing isoforms of pig MYNN, predict the structure and function of coding protein, and study temporal and spatial expression characteristics of each transcript.

Methods

Alternative splice isoforms of MYNN were identified using RNA sequencing (RNA-seq) and cloning techniques. Quantitative real-time PCR (qPCR) was employed to detect expression patterns in 11 tissues of Large White (LW) and Mashen (MS) pigs, and to study developmental expression patterns in cerebellum (CE), stomach (ST) and longissimus dorsi (LD).

Results

The results showed that MYNN had two alternatively spliced isoforms, MYNN-1 (GenBank accession number: KY470829) and MYNN-2 (GenBank accession number: KY670835). MYNN-1 CDS is composed of 1830 bp encoding 609 AA, whereas MYNN-2 CDS is composed of 1746 bp encoding 581 AA. MYNN-2 was 84 bp less than MYNN-1, and lacked the sixth exon. MYNN-2 was found a C2H2 type zinc finger protein domain less than MYNN-1. Two variants were ubiquitously expressed in all pig tissues, and there were significant differences in expression of different tissues (P0.05; P0.01). The expression of MYNN-1 was significantly higher than that of MYNN-2 in almost tissues (P0.05; P0.01), witch testified that MYNN-1 is the main variant. The expression of two isoforms decreased gradually with increase of age in ST and CE of MS pig, whereas increased gradually in LW pig. In LD, the expression of two isoforms increased first and then decreased with increase of age in MS pig, and decreased gradually in LW pig.
Conclusion
Two transcripts of pig MYNN were successfully cloned and MYNN-1 was main variant. MYNN was highly expressed in ST, CE and LD, and their expression were regular. We speculated that MYNN played important roles in digestion/absorption and skeletal muscle growth, whereas the specific mechanisms were still remaining to be further elucidated.
Keywords: pig; MYNN; alternative splicing; mRNA expression
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