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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.18.0025    [Accepted] Published online April 12, 2018.
Expression of Prohibitin, F-actin-capping protein subunit beta and Tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility
Deshun Shi1,*, Xiangping Li1,*, Zhaocheng Xiong1, Haihang Zhang1, Ben Huang1, Qingyou Liu1, Yingqun Wang2
1State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources of Guangxi University, Nanning, China
2Guangxi Livestock and Poultry Variety Reforming Station, Nanning 530001, China
Correspondence:  Deshun Shi, Tel: +86 771 3239202, Fax: +86 771 3239202, Email: ardsshi@gxu.edu.cn
Xiangping Li, Tel: +86 771 3239202, Fax: +86 771 3239202, Email: Xiangpingli@163.com
Received: 5 January 2018   • Revised: 29 January 2018   • Accepted: 5 January 2018
Abstract
Objective: The aim of the current study is to investigate the relationship between PHB, CAPZB, and TEKT2 and sperm motility in Murrah buffalo.

Methods

We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB and TEKT2 in mRNA (message RNA) and protein level.

Results

QRT-PCR (Quantitative Reverse Transcription-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all of the three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both QRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility group compared to the high-motility group (p < 0.05).
Conclusion
The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.
Keywords: Murrah buffaloes; Prohibitin; F-actin-capping protein subunit beta; Tektin-2; Sperm motility
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