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Asian-Australas J Anim Sci > Accepted Articles
DOI: https://doi.org/10.5713/ajas.17.0880    [Accepted] Published online March 13, 2018.
Effects of epigallocatechin-3-gallate on bovine oocytes matured in vitro
Ziqiang Huang1, Yunwei Pang1, Haisheng Hao1, Weihua Du1, Xueming Zhao1, Huabin Zhu1,*
Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China
Correspondence:  Huabin Zhu, Tel: +86-010-62895971, Fax: +86-010-62895971, Email: zhuhuabin@caas.cn
Received: 7 December 2017   • Revised: 18 January 2018   • Accepted: 5 March 2018
Abstract
Objective: Epigallocatechin-3-gallate (EGCG) is a major ingredient of catechin polyphenols and is considered one of the most promising bioactive compounds in green tea because of its strong antioxidant properties. However, the protective role of EGCG in bovine oocyte in vitro maturation (IVM) has not been investigated. Therefore, we aimed to study the effects of EGCG on IVM of bovine oocytes.

Methods

Bovine oocytes were treated with different concentrations of EGCG (0, 25, 50 100, and 200 μM) , and the nuclear and cytoplasmic maturation, cumulus cell expansion, intracellular reactive oxygen species (ROS) levels, total antioxidant capacity, the early apoptosis and the developmental competence of in vitro fertilized embryos were measured. The mRNA abundances of antioxidant genes (NRF2, SOD1, CAT, and GPX4) in matured bovine oocytes were also quantified.

Results

Nuclear maturation which is characterized by first polar body extrusion, and cytoplasmic maturation characterized by peripheral and cortical distribution of cortical granules and homogeneous mitochondrial distribution were significantly improved in the 50 μM EGCG-treated oocytes group compared with the control group. Adding 50 μM EGCG to the maturation medium significantly increased the cumulus cell expansion index, and upregulated the mRNA levels of cumulus cell expansion-related genes (HAS2, TNFAIP6, PTX3, and PTGS2). Both the intracellular ROS level and the early apoptotic rate of matured oocytes were significantly decreased in the 50 μM EGCG group, and the total antioxidant ability was markedly enhanced. Additionally, both the cleavage and blastocyst rates were significantly higher in the 50 μM EGCG-treated oocytes after in vitro fertilization than in the control oocytes. The mRNA abundance of NRF2, SOD1, CAT, and GPX4 were significantly increased in the 50 µM EGCG-treated oocytes.
Conclusion
In conclusion, 50 μM EGCG can improve the bovine oocyte maturation, and the protective role of EGCG may be correlated with its antioxidative property.
Keywords: Epigallocatechin-3-gallate; Reactive oxygen species (ROS); Oocytes; Oxidative Stress; Antioxidant; Bovine


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