Asian-Australas J Anim Sci. Search


Asian-Australas J Anim Sci > Accepted Articles
DOI:    [Accepted] Published online March 13, 2018.
Effects of Xylanase Supplementation to Wheat-based Diets on Growth Performance, Nutrient Digestibility and Gut Microbes in Weanling Pigs
Bing Dong1, Shaoshuai Liu1, Chunlin Wang1, Yunhe Cao1,*
State Key Laboratory of Animal Nutrition, Beijing, China
Correspondence:  Yunhe Cao, Tel: 86-10-62733588, Fax: 86-10-62733688, Email:
Received: 30 November 2017   • Revised: 5 January 2018   • Accepted: 17 February 2018
Objective: This study was designed to investigate the effects of an Aspergillus sulphureus xylanase expressed in Pichia pastoris on the growth performance, nutrient digestibility and gut microbes in weanling pigs.


A total of 180 weanling pigs (initial body weights were 8.47 ± 1.40 kg) were assigned randomly to 5 dietary treatments. Each treatment had 6 replicates with 6 pigs per replicate. The experimental diets were wheat based with supplementation of 0, 500, 1000, 2000 and 4000 U xylanase/kg. The experiment lasted 28 days (early phase, d 0-14; late phase, d 15-28).


In the early phase, compared to the control, average daily gain (ADG) was higher for pigs fed diets supplemented with xylanase and there was a quadratic response in ADG (P<0.05). In the entire phase, ADG was higher for the pigs fed 1000 or 2000 U/kg xylanase compared to the control (P<0.05). The G:F was higher for pigs fed diets supplemented with 1000 or 2000 U/kg xylanase compared to the control (P<0.05). Increasing the amount of xylanase improved the apparent total tract digestibility (ATTD) of dry matter, crude protein, neutral detergent fiber, calcium, and phosphorus during both periods (p<0.05). Xylanase supplementation (2000 U/kg) decreased the proportion of Lachnospiraceae (by 50%) in Firmicutes, but increased Prevotellaceae (by 175%) in Bacteroidetes and almost diminished Enterobacteriaceae (Escherichia-Shigella) in Proteobacteria.
Xylanase supplementation increased growth performance and nutrient digestibility up to 2000 U/kg. Supplementation of xylanase (2000 U/kg) decreased the richness of gut bacteria but diminished the growth of harmful pathogenic bacteria, such as Escherichia-Shigella, in the colon.
Keywords: 16s rRNA sequencing; Bacterial community ; Weanling pigs; Xylanase

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail :               

Copyright © 2018 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next