Asian-Australas J Anim Sci. Search


Asian-Australas J Anim Sci > Accepted Articles
DOI:    [Accepted] Published online December 19, 2017.
Multi-resistance strategy for viral diseases and in vitro shRNA verification method in pigs
Jong-nam Oh1, Kwang-hwan Choi1, C. K Lee1,2,*
1Department of Agricultural Biotechnology, Animal Biotechnology Major, Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Korea
2Designed Animal and Transplantation Research Institute (DATRI), Institute of Green Bio Science and Technology, Seoul National University, Kangwon-do, Korea
Correspondence:  C. K Lee, Tel: 82+2-880-4805, Fax: 82+2-873-2271, Email:
Received: 13 October 2017   • Revised: 21 November 2017   • Accepted: 24 November 2017
Objective: Foot and mouth disease (FMD) and porcine reproductive and respiratory syndrome (PRRS) are major diseases that interrupt porcine production. Because they are viral diseases, vaccinations are of only limited effectiveness in preventing outbreaks. To establish an alternative multi-resistant strategy against FMD virus (FMDV) and PRRS virus (PRRSV), the present study introduced two genetic modification techniques to porcine cells.


First, CD163, the PRRSV viral receptor, was edited with the CRISPR-Cas9 technique. The CD163 gene sequences of edited cells and control cells differed. Second, shRNAs were integrated into the cells. The shRNAs, targeting the 3D gene of FMDV and the ORF7 gene of PRRSV, were transferred into fibroblasts. We also developed an in vitro shRNA verification method with a target gene expression vector.


shRNA activity was confirmed in vitro with vectors that expressed the 3D and ORF7 genes in the cells. Cells containing shRNAs showed lower transcript levels than cells with only the expression vectors. The shRNAs were integrated into CD163-edited cells to combine the two techniques, and the viral genes were suppressed in these cells.
We established a multi-resistant strategy against viral diseases and an in vitro shRNA verification method.
Keywords: Pig; Viral diseases; Foot and mouth disease (FMD); porcine reproductive and respiratory syndrome (PR; CRISPR-Cas9; RNAi
Share :
Facebook Twitter Linked In Google+
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 162 View
  • 0 Download

Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail :               

Copyright © 2018 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next