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Asian-Australas J Anim Sci > Volume 30(11); 2017 > Article
Animal Biotechnology
Asian-Australasian Journal of Animal Sciences 2017;30(11): 1643-1650.
DOI: https://doi.org/10.5713/ajas.17.0689    Published online September 21, 2017.
Comparative analysis of the microbial communities in raw milk produced in different regions of Korea
In Seon Kim1, Yoo Kyung Hur2, Eun Ji Kim2, Young-Tae Ahn3, Jong Geun Kim2,4, Yun-Jaie Choi1,5,*, Chul Sung Huh2,4,*
1Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Korea
2Graduate School of International Agricultural Technology, Seoul National University, Pyeongchang 25354, Korea
3R&BD Center, Korea Yakult Co., Ltd., Yongin 17086, Korea
4Institute of Green-Bio Science & Technology, Seoul National University, Pyeongchang 25354, Korea
5Research Institute for Agriculture and Life Science, Seoul National University, Seoul 08826, Korea
Correspondence:  Yun-Jaie Choi, Tel: +82-2-880-4807, Fax: +82-2-880-7340, Email: cyjcow@snu.ac.kr
Chul Sung Huh, Tel: +82-33-339-5723, Fax: +82-33-339-5855, Email: cshuh@snu.ac.kr
Received: 17 July 2017   • Revised: 18 September 2017   • Accepted: 19 September 2017
Abstract
Objective: The control of psychrotrophic bacteria causing milk spoilage and illness due to toxic compounds is an important issue in the dairy industry. In South Korea, Gangwon-do province is one of the coldest terrains in which eighty percent of the area is mountainous regions, and mainly plays an important role in the agriculture and dairy industries. The purposes of this study were to analyze the indigenous microbiota of raw milk in Gangwon-do and accurately investigate a putative microbial group causing deterioration in milk quality.

Methods

We collected raw milk from the bulk tank of 18 dairy farms in the Hoengseong and Pyeongchang regions of Gangwon-do. Milk components were analyzed and the number of viable bacteria was confirmed. The V3 and V4 regions of 16S rRNA gene were amplified and sequenced on an Illumina Miseq platform. Sequences were then assigned to operational taxonomic units, followed by the selection of representative sequences using the QIIME software package.

Results

The milk samples from Pyeongchang were higher in fat, protein, lactose, total solid, and solid non-fat, and bacterial cell counts were observed only for the Hoengseong samples. The phylum Proteobacteria was detected most frequently in both the Hoengseong and Pyeongchang samples, followed by the phyla Firmicutes and Actinobacteria. Notably, Corynebacterium, Pediococcus, Macrococcus, and Acinetobacter were significantly different from two regions.
Conclusion
Although the predominant phylum in raw milk is same, the abundances of major genera in milk samples were different between Hoengseong and Pyeongchang. We assumed that these differences are caused by regional dissimilar farming environments such as soil, forage, and dairy farming equipment so that the quality of milk raw milk from Pyeongchang is higher than that of Hoengseong. These results could provide the crucial information for identifying the microbiota in raw milk of South Korea.
Keywords: Raw Milk; Microbiota; Milk Quality; Psychrotrophic Bacteria


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