Asian-Australas J Anim Sci. Search

CLOSE


Asian-Australas J Anim Sci > Volume 30(11); 2017 > Article
Environment and Management
Asian-Australasian Journal of Animal Sciences 2017;30(11): 1660-1666.
DOI: https://doi.org/10.5713/ajas.17.0553    Published online September 18, 2017.
Comparison of bacterial communities in leachate from decomposing bovine carcasses
Seung Hak Yang1,*  , Hee Kwon Ahn2, Bong Soo Kim3, Sun Sik Chang1, Ki Yong Chung1, Eun Mi Lee1, Kwang Seok Ki1, Eung Gi Kwon1
1Hanwoo Research Institute, National Institute of Animal Science, Rural Development Administration, Pyeongchang 25340, Korea
2Department of Animal Biosystems Science, Chungnam National University, Daejeon 34134, Korea
3Department of Life Science, Hallym University, Chuncheon 24252, Korea
Correspondence:  Seung Hak Yang, Tel: +82-33-330-0612, Fax: +82-33-330-0660, Email: y64h@korea.kr
Received: 26 July 2017   • Revised: 8 September 2017   • Accepted: 15 September 2017
Abstract
Objective: Burial is associated with environmental effects such as the contamination of ground or surface water with biological materials generated during the decomposition process. Therefore, bacterial communities in leachates originating from the decomposing bovine carcasses were investigated.

Methods

To understand the process of bovine (Hanwoo) carcass decomposition, we simulated burial using a lab-scale reactor with a volume of 5.15 m3. Leachate samples from 3 carcasses were collected using a peristaltic pump once a month for a period of 5 months, and bacterial communities in samples were identified by pyrosequencing of the 16S rRNA gene.

Results

We obtained a total of 110,442 reads from the triplicate samples of various sampling time points (total of 15 samples), and found that the phylum Firmicutes was dominant at most sampling times. Differences in the bacterial communities at the various time points were observed among the triplicate samples. The bacterial communities sampled at 4 months showed the most different compositions. The genera Pseudomonas and Psychrobacter in the phylum Proteobacteria were dominant in all of the samples obtained after 3 months. Bacillaceae, Clostridium, and Clostridiales were found to be predominant after 4 months in the leachate from one carcass, whereas Planococcaceae was found to be a dominant in samples obtained at the first and second months from the other two carcasses. The results showed that potentially pathogenic microbes such as Clostridium derived from bovine leachate could dominate the soil environment of a burial site.
Conclusion
Our results indicated that the composition of bacterial communities in leachates of a decomposing bovine shifted continuously during the experimental period, with significant changes detected after 4 months of burial.
Keywords: Hanwoo; Decomposition; Leachate; Bacterial Community; Firmicutes; Pyrosequencing
TOOLS
Share :
Facebook Twitter Linked In Google+
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 181 View
  • 5 Download


ABOUT
SPECIALTIES
BROWSE ARTICLES
FOR AUTHORS AND REVIEWERS
Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul
08776, Korea   TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2017 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Developed in M2community

Close layer
prev next