1Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences.College of Animal Sciences, Zhejiang University, Hangzhou, China 2State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Zhejiang Academy of Agricultural Sciences, Hangzhou, China 3College of Animal Sciences, Zhejiang University, Hangzhou, China 4Animal Husbandry and Veterinary Institute, Jinhua Academy of Agricultural Sciences, Jinhua, China 5Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou, China
Received: September 30, 2016; Revised: December 16, 2016. Accepted: February 16, 2017. Published online February 23, 2017.
To investigate the effect of host genetics on gut microbial diversity, we performed a structural survey of the fecal microbiota of four purebred boar pig lines: Duroc, Landrace, Hampshire and Yorkshire. The V3-V4 regions of the 16S rRNA genes were amplified and sequenced. A total of 783 OTUs were shared by all breeds, whereas other OTUs were breed-specific. Firmicutes and Bacteroidetes dominated the majority of the fecal microbiota; Clostridia, Bacilli and Bacteroidia were the major classes. Nine predominant genera were observed in all breeds and eight of them can produce short-chain fatty acids (SCFAs). Some bacteria can secrete cellulase to aid fiber digestion by the host. Butyric, isobutyric, valeric and isovaleric acid levels were highest in Landrace pigs, whereas acetic and propionic acid were highest in the Hampshire breed. Heatmap was used to revealed breed-specific bacteria. Principal coordinate analysis (PCoA) of fecal bacteria revealed that the Landrace and Yorkshire breeds had high similarity and were clearly separated from the Duroc and Hampshire breeds. Overall, this study is the first time to compare the fecal microbiomes of four breeds of boar pig by high-throughput sequencing and to use Spearman’s rank correlation to analyze competition and cooperation among the core bacteria.
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