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Asian-Australas J Anim Sci > Volume 30(6); 2017 > Article
Asian-Australasian Journal of Animal Sciences 2017;30(6): 819-827. doi: https://doi.org/10.5713/ajas.16.0589
Characteristics of isolated lactic acid bacteria and their effects on the silage quality
Siran Wang1, Xianjun Yuan1, Zhihao Dong1, Junfeng Li1, Gang Guo2, Yunfeng Bai3, Junyu Zhang1, Tao Shao1,*
1Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, China
2College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China
3Jiangsu Academy of Agricultural Science, Nanjing 210014, China
* Corresponding Author: Tao Shao ,Email: taoshaolan@163.com
Received: August 5, 2016;  Revised: September 11, 2016.  Accepted: October 7, 2016.  Published online October 19, 2016.

Four lactic acid bacteria (LAB) strains isolated from common vetch, tall fescue and perennial ryegrass on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) silage were studied.
The four isolated strains and one commercial inoculant (G, Lactobacillus plantarum MTD­1) were evaluated using the acid production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. The five LAB strains were added to Italian ryegrass for ensiling at three different temperatures (10°C, 15°C, and 25°C).
All isolated strains (LCG3, LTG7, I5, and LI3) could grow at 5°C to 20°C, pH 3.0 to 8.0 and NaCl (3.0%, 6.5%). Strains LCG3, LTG7, I5, and LI3 were identified as Lactobacillus plantarum, Pediococcus acidilactici, Lactobacillus paraplantarum, and Lactobacillus casei by sequencing 16S rDNA, respectively. All LAB inoculants significantly (p<0.05) increased lactic acid (LA) contents and ratios of lactic acid to acetic acid, and reduced pH and ammonia nitrogen/total nitrogen (AN/TN) compared with uninoculated silages at various temperatures (10°C, 15°C, and 25°C). Compared to the commercial inoculant G, I5, and LI3 showed similar effects on improving the silage quality of Italian ryegrass at 10°C and 15°C, indicated by similar pH, LA content and AN/TN.
All inoculants could improve the silage fermentation quality at various temperatures (10°C, 15°C, and 25°C). At the temperature of 10°C and 15°C, strain I5 and LI3 had similar effects with the commercial inoculant G on improving the silage quality of Italian ryegrass.
Keywords: Isolation; Lactic Acid Bacteria; Silage; Low Temperature
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