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Asian-Australas J Anim Sci > Volume 30(6); 2017 > Article
Asian-Australasian Journal of Animal Sciences 2017;30(6): 788-796. doi: https://doi.org/10.5713/ajas.16.0578
Silage preparation and fermentation quality of natural grasses treated with lactic acid bacteria and cellulase in meadow steppe and typical steppe
Meiling Hou1, Ge Gentu1, Tingyu Liu2, Yushan Jia1,*, Yimin Cai3,*
1Laboratory of Grassland Resources, Ministry of Education, P.R. of China, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, Hohhot 010019, China
2College of Agriculture, Inner Mongolia University of Nationalities, Tongliao 028000, China
3Institute of Livestock and Grassland Science, NARO, Tsukuba, Ibaraki 305-0901, Japan
* Corresponding Author: Yushan Jia ,Tel: +86-4714318929, Fax: +86-4714312569, Email: jys_nm@sina.com / Yimin Cai ,Tel: +81-298388648, Fax: +81-298388648, Email: cai@affrc.go.jp
Received: July 27, 2016;  Revised: September 5, 2016.  Accepted: September 20, 2016.  Published online September 27, 2016.
Meiling Hou and Ge Gentu contributed equally to this work.

ABSTRACT
Objective:
In order to improve fermentation quality of natural grasses, their silage preparation and fermentation quality in meadow steppe (MS) and typical steppe (TS) were studied.
Method:
The small-scale silages and round bale silages of mixed natural grasses in both steppes were prepared using the commercial lactic acid bacteria (LAB) inoculants Chikuso-1 (CH, Lactobacillus plantarum) and cellulase enzyme (AC, Acremonium cellulase) as additives.
Results:
MS and TS contained 33 and 9 species of natural grasses, respectively. Stipa baicalensis in MS and Stipa grandi in TS were the dominant grasses with the highest dry matter (DM) yield. The crude protein (CP), neutral detergent fiber and water-soluble carbohydrate of the mixed natural grasses in both steppes were 8.02% to 9.03%, 66.75% to 69.47%, and 2.02% to 2.20% on a DM basis, respectively. All silages treated with LAB and cellulase were well preserved with lower pH, butyric acid and ammonia-N content, and higher lactic acid and CP content than those of control in four kinds of silages. Compared with CH- or AC-treated silages, the CH+ AC-treated silages had higher lactic acid content.
Conclusion:
The results confirmed that combination with LAB and cellulase may result in beneficial effects by improving the natural grass silage fermentation in both grasslands.
Keywords: Meadow Steppe; Natural Grass; Silage Fermentation; Typical Steppe
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