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Asian-Australas J Anim Sci > Volume 30(6); 2017 > Article
Asian-Australasian Journal of Animal Sciences 2017;30(6): 878-885. doi: https://doi.org/10.5713/ajas.16.0515
Mammary alveolar cell as in vitro evaluation system for casein gene expression involved in glucose level
Young Tae Heo1, Woo Tae Ha1, Ran Lee1, Won-Young Lee2, Ha Yeon Jeong3, Kyu Chan Hwang4, Hyuk Song1,*
1Department of Stem Cell and Regenerative Biology, College of Animal Bioscience and Technology, Konkuk University, Seoul 143-701, Korea
2Division of Food Bioscience, RIBHS, College of Biomedical and Health Sciences, Konkuk University, Chung-ju 380-701, Korea
3Department of Animal Resources Development Dairy Science Division, National Institute of Animal Science, RDA, Cheonan 331-801, Korea
4Sooam Biotech Research Foundations, Seoul 152895, Korea
* Corresponding Author: Hyuk Song ,Tel: +82-2-2450-0562, Email: songh@konkuk.ac.kr
Received: July 5, 2016;  Revised: August 8, 2016.  Accepted: September 19, 2016.  Published online September 19, 2016.
Young Tae Heo and Woo Tae Ha contributed equally to this work.

ABSTRACT
Objective:
Glucose is an essential fuel in the energy metabolism and synthesis pathways of all mammalian cells. In lactating animals, glucose is the major precursor for lactose and is a substrate for the synthesis of milk proteins and fat in mammary secretory (alveolar) epithelial cells. However, clear utilization of glucose in mammary cells during lactogenesis is still unknown, due to the lack of in vitro analyzing models. Therefore, the objective of this study was to test the reliability of the mammary alveolar (MAC-T) cell as an in vitro study model for glucose metabolism and lactating system.
Method:
Undifferentiated MAC-T cells were cultured in three types of Dulbecco's modified Eagle's medium with varying levels of glucose (no-glucose: 0 g/L, low-glucose: 1 g/L, and high-glucose: 4.5 g/L) for 8 d, after which differentiation to casein secretion was induced. Cell proliferation and expression levels of apoptotic genes, Insulin like growth factor-1 (IGF1) receptor, oxytocin receptor, αS1, αS2, and β casein genes were analyzed at 1, 2, 4, and 8 d after differentiation.
Results:
The proliferation of MAC-T cells with high-glucose treatment was seen to be significantly higher. Expression of apoptotic genes was not affected in any group. However, expression levels of the mammary development related gene (IGF1 receptor) and lactation related gene (oxytocin receptor) were significantly higher in the low-glucose group. Expressions of αS1-casein, αS2-casein, and β-casein were also higher in the low-glucose treated group as compared to that in the no-glucose and high-glucose groups.
Conclusion:
The results demonstrated that although a high-glucose environment increases cell proliferation in MAC-T cells, a low-glucose treatment to MAC-T cells induces higher expression of casein genes. Our results suggest that the MAC-T cells may be used as an in vitro model to analyze mammary cell development and lactation connected with precise biological effects.
Keywords: Glucose; Mammary alveolar (MAC-T) Cell; Insulin-like Growth Factor-1 (IGF-1); Oxytocin; Casein
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