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Asian-Australas J Anim Sci > Volume 29(10); 2016 > Article
Long, Wu, Yuan, Wang, Zhang, and Qi: Effects of Dietary Octacosanol on Growth Performance, Carcass Characteristics and Meat Quality of Broiler Chicks


Octacosanol, which has prominent physiological activities and functions, has been recognized as a potential growth promoter in animals. A total of 392 1-d-old male Arbor Acres broiler chicks with similar body weight were randomly distributed into four dietary groups of seven replicates with 14 birds each supplemented with 0, 12, 24, or 36 mg octacosanol (extracted from rice bran, purity >92%)/kg feed. The feeding trial lasted for six weeks and was divided into the starter (day 1 to 21) and the grower (day 22 to 42) phases. The results showed that the feed conversion ratio (FCR) was significantly improved in broilers fed a diet containing 24 mg/kg octacosanol compared with those fed the control diet in the overall phase (day 1 to 42, p = 0.042). The average daily gain and FCR both showed linear effects in response to dietary supplementation of octacosanol during the overall phase (p = 0.031 and 0.018, respectively). Broilers fed with 24 or 36 mg/kg octacosanol diet showed a higher eviscerated yield, which increased by 5.88% and 4.26% respectively, than those fed the control diet (p = 0.030). The breast muscle yield of broilers fed with 24 mg/kg octacosanol diet increased significantly by 12.15% compared with those fed the control diet (p = 0.047). Eviscerated and breast muscle yield increased linearly with the increase in dietary octacosanol supplementation (p = 0.013 and 0.021, respectively). Broilers fed with 24 or 36 mg/kg octacosanol diet had a greater (p = 0.021) pH45min value in the breast muscle, which was maintained linearly in response to dietary octacosanol supplementation (p = 0.003). There was a significant decrease (p = 0.007) in drip loss value between the octacosanol-added and the control groups. The drip loss showed linear (p = 0.004) and quadratic (p = 0.041) responses with dietary supplementation of octacosanol. These studies indicate that octacosanol is a potentially effective and safe feed additive which may improve feed efficiency and meat quality, and increase eviscerated and breast muscle yield, in broiler chicks. Dietary supplementation of octacosanol at 24 mg/kg diet is regarded as the recommended dosage in the broilers’ diet.


Octacosanol (HO-CH2-[CH2]26CH3), a long-chain aliphatic alcohol, is the main component of a natural wax product that exists in wheat germ oil, rice bran oil, fruits or leaves (Taylor et al., 2003; de Oliveira et al., 2012). Octacosanol has been reported to exhibit a variety of important biological activities in humans and rodents, including antifatigue properties (Kim et al., 2004), antioxidant activities (Ohta et al., 2008), cholesterol-lowering effects (Hernandez et al., 1992), cytoprotective function (Carbajal et al., 1996) and ergogenic properties (Oliaro-Bosso et al., 2009). Studies have shown that the addition of dietary octacosanol has a beneficial effect on metabolic responses to submaximal cycle ergometry, grip and chest strength, and reaction time in human subjects (Saint-John and McNaughton, 1986), and has enhanced the running performance and related biochemical parameters in exercise-trained rats (Kim et al., 2004). Other studies on the effects of octacosanol on lipid metabolism have revealed that the addition of octacosanol leads to a significant decrease in the weight of perirenal adipose tissue of high-fat diet rats (Taylor et al., 2003) and reduces low-density lipoprotein (LDL), triacylglycerol and cholesterol content in the plasma of mice, healthy volunteers or patients (Aneiros et al., 1995; Menendez et al., 2000; Xu et al., 2007). Decreased cholesterol synthesis or enhanced LDL catabolism may be the mechanism of action here (Gouni-Berthold and Berthold, 2002). In terms of antioxidant function, octacosanol protects against CCl4-induced liver injury in rats by attenuating disrupted hepatic reactive oxygen species metabolism (Ohta et al., 2008), and inhibits in vitro copper ion-induced rat lipoprotein peroxidation (Menendez et al., 1999). In addition, cytoprotection and anti-inflammatory properties have also been confirmed (Carbajal et al., 1996; de Oliveira et al., 2012). Mixtures of long-chain aliphatic alcohols (e.g. policosanol, of which octacosanol is the main component) have been shown to exert similar effects (Arruzazabala et al., 1994; Singh et al., 2006). These studies on the physiological function of octacosanol or policosanol were mainly conducted on rodents or human volunteers.
In view of its prominent physiological functions and high level of safety, with LD50>18,000 mg/kg (Pons et al., 1993), octacosanol has been widely applied to health food, functional beverage, medicine and other fields. Due to its versatile functions, octacosanol may be developed as a potential feed additive for domestic animals. In our previous study, we found that octacosanol increased the growth performance of weaning piglets (Long et al., 2015), which indicated that the addition of octacosanol was effective in animal production. The objective of this study was, therefore, to test the efficacy of dietary octacosanol supplementation on growth performance, carcass characteristics and meat quality in broiler chicks, offering the poultry industry a new direction.


Birds and management

The animal protocol for the present study was approved by the Animal Care and Use Committee of Feed Research Institute of the Chinese Academy of Agricultural Sciences. A total of 392 1-d-old male Arbor Acres broiler chicks with similar body weight (BW) was obtained from the Beijing Huadu Broiler Company (Beijing, China). All chicks were housed in a standardized chicken house with double-floor cages containing a tube feeder, a nipple drinker line and built-up soft-wood shavings, and exposed to a 23 h of light for the first two weeks and 20 h of light thereafter. The initial temperature in the chicken house was 34°C for the first week and was reduced by 2°C each consecutive week until the temperature reached 24°C. Relative humidity was set at 50% throughout the trial. Diets, in pellet form, and fresh water were supplied ad libitum, and ventilation was controlled. All management of broilers was in accordance with the recommendations of the Arbor Acres Broiler Management Handbook (Aviagen Inc., 2014).

Experimental design and the diets

The broilers were randomly divided into four groups with seven replicates of 14 birds each. Broilers were fed with a basal diet (Table 1) supplemented with 0, 12, 24, or 36 mg octacosanol/kg diet. Octacosanol (purity>92%), extracted from rice bran, was provided by the Huzhou Shuanglin Shengtao Vegetable Oil Factory (Zhejiang, China). Basal diets, divided into two phases according to the ages of the chicks (starter phase, 1 to 21 d; grower phase, 22 to 42 d), were formulated according to the NRC (1994) and the Chinese Feeding Standard of Chicken (Ministry of Agriculture of China, 2004).

Growth performance

The data relating to the BW and feed intake of the chicks during the starter phase (d 1 to 21) and the grower phase (d 22 to 42) were recorded. The average daily gain (ADG), average daily feed intake (ADFI) and feed conversion ratio (FCR, the ratio of feed to gain) were calculated for each period and cumulatively. These parameters were corrected according to mortality.

Carcass characteristics

On the last day of the experiment, two chicks were selected from each replicate and killed after a 12 h fasting for slaughter mensuration. The sacrificed chicks were exsanguinated and deplumed immediately. Next, the eviscerated weight and the internal organ weight of the heart, liver and spleen were measured. Breast muscles (including pectoralis major and minor), leg muscles (including thigh and drumstick muscles) and abdominal fat (including leaf fat surrounding the cloaca and abdominal fat surrounding the gizzard) were removed and weighed. Eviscerated yield, breast muscle yield, leg muscle yield, abdominal fat percentage and relative internal organ weight were all calculated as percentages of BW. The average value of the two broilers was regarded as the replicate value.

Meat quality

The qualities of the breast muscle were evaluated. The meat colour at three points of a meat piece was measured with a spectrocolorimeter (Minolta-CR200, Tokyo, Japan) at 45 min after slaughter. The meat quality was evaluated according to the Commission Internationale de L’Eclairage (CIE) L*a*b* coordinates (where L* indicates relative lightness, a* indicates relative redness, and b* indicates relative yellowness). At the same time, the pH values at a depth of 2.5 cm below the surface for each sample were measured at 45 min, as initial pH, and at 24 h, as ultimate pH, after slaughter using a pH meter (Star A, Thermo Fisher Scientific, Waltham, MA, USA). Next, drip loss was measured using approximately 2 g of breast muscle sample according to the method described by Honikel (1998).

Statistical analysis

All data were performed using SPSS 17.0 for Windows software and expressed as mean values with pooled standard errors of the means using one-way analysis of variance. The difference between group means was separated by the least significant difference (Duncan’s) multiple range test. The dose-response effect of supplemental octacosanol was computed using orthogonal polynomial contrast for linear and quadratic effects. Differences were considered statistically significant at p<0.05 unless otherwise stated.


Growth performance

The results of growth performance of broilers are presented in Table 2. No significant differences in growth performance were observed between the octacosanol-added and control groups during the starter phase (d 1 to 21, p>0.05). In the grower phase (d 22 to 42), dietary supplementation of octacosanol improved BW and FCR compared with the control group, and BW increased linearly (p = 0.031) with dietary supplementation of octacosanol, while FCR decreased linearly (p = 0.016). During the overall phase (d 1 to 42), chicks fed with 24 mg/kg octacosanol diet showed comparably lower FCR, which decreased by 5.17%, compared with those fed the control diet (p = 0.042). Average daily gain and FCR both showed linear effects in response to dietary addition of octacosanol during the overall phase (p = 0.031 and 0.018, respectively). No significant difference was observed in ADFI among all groups (p>0.05).

Carcass characteristics

The effect of dietary supplementation of octacosanol on the carcass characteristics of broilers is presented in Table 3. Broilers fed with dietary supplemental 24 or 36 mg/kg octacosanol showed higher eviscerated yield, which increased by 5.88% and 4.26% respectively, than those fed the control diet (p = 0.030). Compared with the control group, breast muscle yield of the broilers was significantly increased by 12.15% when the broilers were fed with 24 mg/kg octacosanol diet (p = 0.047). There was a linear increase in eviscerated (p = 0.013) and breast muscle yield (p = 0.021) with the dietary addition of octacosanol. No differences were observed in leg muscle yield and abdominal fat percentage among different groups (p>0.05). However, the abdominal fat content decreased linearly in response to dietary octacosanol supplementation (linear effect, p = 0.048). No significant differences were found in the heart, liver or spleen weight among all groups (p>0.05).

Meat quality

Broilers fed with the 24 or 36 mg/kg octacosanol diets had a higher pH45min value (p = 0.021) in the breast muscle, which remained higher linearly—in response to the dietary addition of octacosanol (p = 0.003)—than those fed the control diet (Table 4). No differences were observed in pH24h value among all treatments (p>0.05) or in L*, a*, or b* value among all treatments (p>0.05). However, the a* value increased linearly in response to dietary octacosanol supplementation (linear effect, p = 0.051). In addition, a significant difference (p = 0.007) in drip loss was observed between the octacosanol-added groups and the control group. Compared with the control diet, the chicken drip loss decreased by 2.97%, 5.03%, and 3.89%, which showed linear (p = 0.004) and quadratic (p = 0.041) effects with dietary supplementation of octacosanol.


Octacosanol or policosanol is considered to be a safe agent (Arruzazabala et al., 1994). As it is an additive, the effect on the growth performance of animals is the most valuable index. This study revealed that the dietary addition of octacosanol improved the growth performance of broiler chicks and that the dietary addition of octacosanol at 24 mg/kg diet showed the best feed efficiency during the overall period. Xu and Shen (1997) obtained the same results by supplementing the diet of broiler chicks with 25 mg/kg octacosanol for 42 days. In addition, the study by Xu et al. (2007) reported that a 1% octacosanol (wt/wt) diet group of E-KO mice gained weight at a rate comparable with the control group, while the study by Xiang et al. (2012) revealed that moderate doses of octacosanol in rats effectively increase the ADFI and ADG, and improve feed efficiency. These results support the hypothesis that octacosanol may improve the growth performance of domestic animals.
Other studies have shown that dietary octacosanol supplementation effectively promotes the secretion of growth hormone (GH) in the blood of rats (Yang, 2012), which can promote protein deposition and bone growth by regulating metabolic processes and energy metabolism in the body (Brown-Borg and Bartke, 2012). Moreover, octacosanol is effective in modulating lipid metabolism, in enhancing glycogen storage, and in decreasing the rate of glycogen utilization in muscle (Kato et al., 1995; Kim et al., 2004), which is due in part to increased energy metabolism through the activation of adenosine monophosphate-activated protein kinase (AMPK) in the muscle (Oliaro-Bosso et al., 2009). These findings indicate that octacosanol may potentially be used as an ergogenic agent. Accordingly, one possible reason for the improvement in the broilers’ growth performance with octacosanol is the resulting increase in GH levels in the chicks along with an enhanced rate of glycogen utilization for growth (Kim et al., 2004). Further research is needed to clarify the effect of octacosanol on GH in broilers’ blood and the efficacy of octacosanol as an ergogenic compound.
To our knowledge, the effects of dietary supplementation of octacosanol on carcass characteristics have not yet been reported. In our current study, octacosanol improved the eviscerated yield and breast muscle yield of broilers. Moreover, it led to a decrease in abdominal fat content. Octacosanol was also shown to be effective in improving the carcass characteristics of broilers.
In fast-growing broilers, growth occurs primarily in the pectorals and feathers during the late stages of development (Scheele, 1997). The relative increase in growth in octacosanol-fed chicks in the grower phase may partly contribute to the comparable increase in breast muscle. Published studies have reported that octacosanol may stimulate the mRNA expression of glycogen synthase in muscle (Yang, 2012), which is effective in regulating glycogen supply and in maintaining the energy balance in muscle. The major metabolic consequences of adaptation to physical functions are a slower rate of muscle glycogen utilization and a greater reliance on fat oxidation in the muscle (Crowley et al., 1996). Thus, in our study, more energy and nutrients were directed towards growth, e.g. breast development, when octacosanol was added to the diet, which improved the feed utilization by broilers during the grower stage. In contrast, no effects on leg muscle yield were observed with dietary supplementation of octacosanol. This result may be explained by the different compositions of muscle fibre types, the rate of protein turnover (Baillie and Garlick, 1991) and the differences in chemical composition (Douris et al., 2006; Lavery et al., 2008) between the breast and leg muscles of animals. In addition, it has been reported that muscle growth factors are more effective in breast muscles than in leg muscles (Qiao et al., 2013). This effect of octacosanol on muscle tissues in broilers is worthy of follow-up investigation.
In the present study, the abdominal fat percentage decreased in response to the increase in octacosanol. Kato et al. (1995) also showed that dietary octacosanol supplementation significantly reduced the weight of perirenal adipose tissue in high-fat diet rats. Moreover, other studies have reported that the development of adipose tissue depends on the availability of serum lipids, which are principal substrates in lipid metabolism (Zhang et al., 2011; Liao et al., 2015). Accordingly, the decrease in abdominal fat content evoked by octacosanol supplementation may be associated with the effect of serum lipid metabolism and the inhibition of cholesterol synthesis (Shimura et al., 1987; Hernandez et al., 1992; Arruzazabala et al., 1994; Castano et al., 2000). Additional experiments are needed to confirm the effect of octacosanol on lipid metabolism.
Research into the influence of octacosanol on muscle qualities, such as fibre size, density, and transformation, remains limited. In this study, we investigated the effect of octacosanol on the meat quality of animals. It is demonstrated that the addition of octacosanol influences pH45min, a* values and drip loss. Glycolysis—anaerobic metabolism initiated in post-mortem breast muscle—can produce lactic acid and lead to a reduction in meat pH (Cai et al., 2015), which will influence the colour and water-holding capacity of chicken. In addition, meat colour, which mainly depends on the content of the muscle myoglobin, is a comprehensive index reflecting muscle physiology and biochemical and microbiological changes. Drip loss is a sensitive index of muscle protein structure and charge change, which directly influence the flavour, tenderness, processing and storage of the muscle. An improvement in pH45min, a* values and drip loss results in improved quality. The mechanisms for the improvement in meat quality concomitant with dietary octacosanol supplementation remain unclear, but it has been presumed that the effect may be attributed to the antioxidant properties of octacosanol.
Some studies have suggested that preventing lipid peroxidation in intramuscular fat—which is rich in unsaturated fatty acids and easily oxidized, and has phospholipids as its main component—is beneficial to maintaining the quality and flavour of meat (McCormick, 1999; Chikunya et al., 2004; Kamboh and Zhu, 2013). Studies have confirmed that octacosanol can inhibit both liver and blood lipid oxidation (Ohta et al., 2008; Long et al., 2015) and protect lipoprotein particles against lipid peroxidation in animals (Menendez et al., 1999; Yu, 2003). Therefore, one reason for the improvement in meat quality produced by octacosanol may be the antioxidant effect. The exact mechanism, however, needs further elucidation.


In summary, the results of the present study indicate that octacosanol is an effective and safe feed additive as it can improve feed efficiency, stimulate eviscerated yield and breast muscle yield, and contribute to good meat quality. In this study, dietary supplementation of octacosanol at 24 mg/kg was regarded as the recommended dosage in the diet of broilers.


The financial support provided by the China Agriculture Research System-Beijing Team for Poultry Industry and the Agricultural Science and Technology Innovation Program (ASTIP) is gratefully appreciated.



We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

Table 1
Dietary composition and nutrient levels of the basal diets (as-fed basis)
Starter phase (d 1 to 21) Grower phase (d 22 to 42)
Ingredient (%)
 Corn 60.80 63.90
 Soybean meal 32.90 28.95
 Soybean oil 2.00 3.00
 Dicalcium phosphate 1.62 1.50
 Limestone 1.55 1.60
 Salt 0.36 0.36
 DL-methionine 0.23 0.16
 L-lysine·HCl 0.25 0.24
 Vitamin-mineral premix1 0.29 0.29
 Total 100.00 100.00
Nutrient level2
 AME (MJ/kg) 12.12 12.50
 CP (%) 20.30 (20.85) 18.56 (18.02)
 Ca (%) 0.96 (1.02) 0.94 (0.86)
 Total P (%) 0.64 (0.68) 0.60 (0.62)
 Available P (%) 0.41 0.39
 Lysine (%) 1.10 1.00
 Methionine (%) 0.52 0.41
 Methionine+cysteine (%) 0.83 0.72

AME, apparent metabolizable energy; CP, crude protein.

1 Supplied per kg diet: vitamin A, 12,500 IU; vitamin D3, 2,500 IU; vitamin E, 15 IU; vitamin K3, 2.5 mg; vitamin B1, 3.2 mg; vitamin B2, 6.5 mg; vitamin B12, 0.025 mg; nicotinic acid, 30 mg; calcium pantothenate, 12 mg; biotin, 0.03 mg; folic acid, 1.0 mg; iron, 80 mg; copper, 8 mg; manganese, 100 mg; zinc, 75 mg; iodine, 0.35 mg; and selenium, 0.15 mg.

2 The value in parentheses indicates the analyzed value. Others are calculated values.

Table 2
Effect of dietary octacosanol supplementation on growth performance of broilers1
Items Dietary octacosanol addition (mg/kg) Pooled SEM p-value

0 12 24 36 ANOVA2 Linear3 Quadratic3
Starter phase (d 1 to 21)
 BW d 21 (g) 729 725 740 731 3.22 0.450 0.458 0.719
 ADG (g) 32.62 32.44 33.14 32.74 0.15 0.445 0.453 0.716
 ADFI (g) 48.83 48.07 48.39 49.28 0.52 0.874 0.742 0.458
 FCR (feed:gain, g:g) 1.496 1.483 1.460 1.506 0.02 0.815 0.964 0.414
Grower phase (d 22 to 42)
 BW d 42 (g) 2,272 2,310 2,351 2,339 12.52 0.114 0.031 0.302
 ADG (g) 73.50 75.47 76.71 76.55 0.61 0.222 0.060 0.374
 ADFI (g) 144.95 145.67 142.29 144.04 0.69 0.349 0.325 0.709
 FCR (feed:gain, g:g) 1.973 1.935 1.857 1.877 0.02 0.061 0.016 0.370
Whole phase (d 1 to 42)
 ADG (g) 53.07 53.96 54.94 54.64 0.30 0.111 0.031 0.298
 ADFI (g) 96.90 96.86 95.34 96.41 0.39 0.486 0.409 0.488
 FCR (feed:gain, g:g) 1.829b 1.796ab 1.737a 1.764ab 0.01 0.042 0.018 0.188

SEM, standard error of the mean; ANOVA, analysis of variance; BW, body weight; ADG, average daily gain; ADFI, average daily feed intake; FCR, feed conversion ratio.

1 n = 7 per treatment, with 14 broilers per replicate.

2 One-way ANOVA of all treatment groups.

3 Linear and quadratic effects of increasing inclusion levels of octacosanol.

a,b Values within a row with different superscript letters are significantly different (p<0.05).

Table 3
Effect of dietary octacosanol supplementation on carcass characteristics of broilers at d 421
Items2 Dietary octacosanol addition (mg/kg feed) Pooled SEM p-value

0 12 24 36 ANOVA3 Linear4 Quadratic4
Eviscerated yield (%) 70.70b 72.55ab 74.86a 73.71a 0.54 0.030 0.013 0.126
Breast muscle yield (%) 18.03b 19.11ab 20.22a 19.62ab 0.29 0.047 0.021 0.126
Leg muscle yield (%) 15.98 16.54 16.86 16.34 0.24 0.662 0.543 0.293
Abdominal fat (%) 1.77 1.73 1.70 1.70 0.01 0.201 0.048 0.437
Heart weight (g/kg BW) 4.92 4.66 4.51 4.99 0.08 0.098 0.929 0.081
Liver weight (g/kg BW) 16.19 16.56 16.37 16.82 0.19 0.702 0.340 0.911
Spleen weight (g/kg BW) 1.05 1.06 1.04 1.09 0.01 0.562 0.445 0.557

SEM, standard error of the mean; ANOVA, analysis of variance; BW, body weight.

1 n=7 per treatment, with 2 birds per replicate.

2 All the indicators were calculated as percentage of BW.

3 One-way ANOVA of all treatment groups.

4 Linear and quadratic effects of increasing inclusion levels of octacosanol.

a,b Values within a row with different superscript letters are significantly different (p<0.05).

Table 4
Effect of dietary octacosanol supplementation on meat quality of broilers at d 421
Items Dietary octacosanol addition (mg/kg feed) Pooled SEM p-value

0 12 24 36 ANOVA2 Linear3 Quadratic3
pH45min4 6.04b 6.13ab 6.15a 6.20a 0.02 0.021 0.003 0.560
pH24h4 5.72 5.72 5.70 5.79 0.02 0.345 0.261 0.243
Lightness (L*) 57.29 57.34 57.18 57.69 0.11 0.434 0.312 0.318
Redness (a*) 13.78 14.34 14.76 14.42 0.14 0.083 0.051 0.095
Yellowness (b*) 14.70 14.51 14.43 15.00 0.18 0.698 0.623 0.308
Drip loss5 (%) 4.37B 4.24A 4.15A 4.20A 0.02 0.007 0.004 0.041

SEM, standard error of the mean; ANOVA, analysis of variance.

1 n = 7 per treatment, with 2 birds per replicate.

2 One-way ANOVA of all treatment groups.

3 Linear and quadratic effects of increasing inclusion levels of octacosanol.

4 pH45min, 45 min postmortem; pH24h, 24 h postmortem.

5 Measured when hung at 48 h.

a,b Values within a row with different superscript letters are significantly different (p<0.05).

A,B Values within a row with different superscript letters differ significantly (p<0.01).


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