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Asian-Australas J Anim Sci > Volume 29(8); 2016 > Article
Seong, Seo, Kang, Kim, Cho, Kim, and Hoa: Beneficial Effects of Traditional Seasonings on Quality Characteristics of Fermented Sausages


Though traditional seasonings are widely used in many dishes, however, no attention has been paid to the investigation of their effects on quality characteristics of food products. The present investigation was undertaken to study the effects of incorporating several traditional seasonings including doenjang (fermented soybean paste), gochu-jang (red pepper paste), fresh medium-hot, and hot peppers, and fresh garlic on the lipid oxidation, cholesterol content and sensory characteristics of fermented sausages. Six fermented sausage treatments (5 with 1% (w/w) each test seasoning and 1 without added test seasoning (control) were prepared. The addition of seasonings generally had beneficial effects on the improvement of fermented sausage’s quality however the effects differed depending on the each type of seasonings added. Significant lower pH values were found in all fermented sausages made with the seasonings while, lower levels of lipid oxidation were found in the treatments with hot peppers and garlic as compared with the control (p<0.05). The treatment with seasonings did not cause color or texture defects in the products whereas the sausages made with gochu-jang had significantly higher Commission International de l’Eclairagea* (redness) value in comparison with the control. Noticeably, incorporating doenjang, medium-hot peppers, hot peppers and garlic resulted in reduction of 26.50, 32.54, 47.04, and 48.54 mg cholesterol/100 g samples, respectively (p<0.05). Higher scores for the sensory traits such as aroma, taste, color and acceptability were also given for the sausages made with seasonings. The current work demonstrates that the test seasonings represent potentially natural ingredients to be used for producing healthier fermented sausages.


Fermented sausages are generally manufactured with about 60% to 80% raw meat, 20% to 40% fat and along with additives and spices etc. (Nassua et al., 2003; Kurćubić et al., 2014). The fermented sausages are a rich source of high biological value proteins, energy and minerals. Moreover, the products are generally produced without any heat treatment throughout the process of fermentation, ripening and drying, which enables nutritional values to remain unchanged. However, the products also have some negative aspects such as unfavorable polyunsaturated/saturated fatty acid ratio and an especially high cholesterol level due to the high added fat level (Muguerza et al., 2001). Besides, the products are highly susceptible to lipid oxidation which generates toxic molecules with possible hazard for human health as well as reducing shelf-life stability of the product (Zanardi et al., 2004). Therefore, the control of lipid oxidation and reducing the cholesterol content are necessary to improve the shelf-life stability and health effect of fermented sausages. Despite lipid oxidation in meat products being controlled or minimized, by the addition of commercial synthetic antioxidants, the available synthetic antioxidants (e.g., butylated hydroxyanisole and butylated hydroxytoluene) have been suspected to cause toxicity problems that negatively affect consumer’s health. Therefore, these compounds have had their use restricted in foods (Botterweck et al., 2000). Consumers are increasingly aware of the importance of their food choices in health maintenance and disease prevention, therefore, a new trend to substitute these synthetic additives with natural functional ingredients has received serious attention from consumers and meat processors (Nassua et al., 2003; Kurćubić et al., 2014; Chaves-López et al., 2015). In addition, the incorporation of natural additives into the conventional processing formulations makes the meat products not only healthier but also improves sensory quality (Ozvural and Vural, 2011; Seong et al., 2015).
Doenjang, a soybean fermented product, has long been used as a healthy food and seasoning in Korea and this kind of product is also widely used in other countries such as China and Japan etc. The doenjang is a rich source of protein, carbohydrates, unsaturated fatty acids and soluble fiber, and more importantly it contains a significant level of bioactive compounds which exert a potent biological activity such as antioxidant, antimicrobial, anticancer and anti-obesity effects (Kim et al., 2005; Cha et al., 2014; Shukla et al., 2014). Like the doenjang, gochu-jang, a kind of red pepper paste made from dried pepper powder, water, salt, fermented soybean and sweet rice powder, is also commonly used as a seasoning in many dishes in Korea (Kwon et al., 2015). Besides, the other plant-sourced seasonings such as fresh chili peppers and garlic are also widely used not only for their ability to enhance the typical flavor of dishes or food products but also for their beneficial effects on health such as reducing cholesterol level in blood and cardiovascular risk (Hermansen et al., 2003).
In previous studies, authors have demonstrated that the addition of natural ingredients such as mushroom powder could significantly improve the sensory quality (e.g., taste, flavor and overall acceptability) of meat products such as frankfurters (Seong et al., 2015). While the traditional seasonings from natural sources like doenjang, gochu-jang, fresh garlic and chili peppers are abundant, inexpensive and widely used for multi-purposes as mentioned above, no attention has been paid to the effects of their addition on the quality of meat products. Therefore, the objective of the present study was to assess the impacts of incorporation of some traditional seasonings on the technological quality characteristics, cholesterol content and sensory perception of fermented sausages.



Pork ham and back-fat were obtained from a local slaughterhouse (Suwon, Korea) 24 h after slaughter. Korean traditional seasonings including doenjang and gochu-jang, and natural seasonings such as fresh green medium-hot peppers, green hot peppers and fresh garlic cloves were purchased from a local market in Suwon, South Korea. The other ingredients used were sodium nitrite and sodium nitrate (Sigma Chemical Co. St. Louis, MO, USA), polyphosphate and sodium ascorbate (Shinyo Pure Chemicals Co. Osaka, Japan), sodium chloride (Hanju Co. Ulsan, Korea), sugar and black pepper (Beksul Food Co. Seoul, Korea).

Fermented sausage formulation and processing

Fresh peppers (both medium-hot and hot types) and garlic cloves were washed to remove any impurities, left to drain the water, cut and then ground separately using a blender (HMF 3160S, Hanil Electric, Co, Seoul, Korea). The initial pH values of goenjang, gochu-jang, ground medium-hot peppers (GMP), ground hot peppers (GHP) and ground garlic (GG) were 5.70, 5.45, 5.65, 5.63, and 5.80, respectively.
Six different treatments of dry fermented sausages (20 kg each) were prepared in the present study. All sausage treatments (T) were formulated with 80% pork ham, 20% pork backfat, 2% sodium chloride, 1.5% sugar, 0.2% black pepper, 0.2% polyphosphate, 0.03% sodium ascorbate, 0.01% sodium nitrite and 0.005% sodium nitrate. Besides, 1% (w/w) of each test seasonings such as doanjang, gochu-jang, GMP, GHP, and GG was added to the T1, T2, T3, T4, and T5, respectively. The batch made without the added test seasonings served as the control. The processing procedure of fermented sausages was performed as follows: The refrigerated meat (48 h after slaughter) was first trimmed of all connective tissue and visible fats and then cut into small pieces. The trimmed meat and pork back-fat were chopped through a 0.5 cm plate using a silent chopper (Model 7548, Biro MFG. Co, Marblehead, OH, USA). The chopped meat was then mixed with all of the ingredients (except for the test seasonings) and left in a chilling room for overnight. The following day, for each treatment, the chopped meat and fat were placed in a meat tumbler and mixer (model CE93, RUHLE GMBH, Grafenhausen, Germany) and then the test seasoning was also added to the corresponding treatment and mixed for further 15 min at 4°C. After mixing, the meat batters were immediately stuffed into 110-mm diameter collagen casings (Naturin Viscofan Co, Navarra, Spain) using a vacuum stuffer (Model VF610, Handtmann Co, Biberach, Germany), resulting in sausages of ca. 1.5 kg. The filled sausages were then hung vertically on metal rods, set on the cart and transferred to an air-conditioned chamber for fermenting, ripening and drying. Temperature and relative humidity (RH) conditions in the air-conditioned chamber during fermentation, ripening and drying were set as follows: 15°C/90% to 100% RH for 18 h, 22°C/90% RH for 48 h, 14°C to 15°C/80% to 90% RH for 10 days, 14°C/75% RH for 28 days. At the end of the ripening/drying (42 days), the fermented sausages were collected and stored at 4°C until use.
For the sample preparation, the outer casings were removed, and each fermented sausage was cut into sub-sample sizes depending on type of analysis. The samples used for analyses of proximate composition, pH, lipid oxidation and cholesterol and sodium were ground with a meat blender, vacuum-packed in polyethylene bags and stored at −20°C until analysis, while the samples for the sensory evaluation was used on the same sample preparation day.

Proximate composition

Moisture, fat and protein contents were analyzed using the method of the Association of Official Analytical Chemists (AOAC, 2000). Particularly, the moisture and fat contents were determined by using a Food Scan Lab 78810 (Foss Tecator Co., Ltd., Hillerod, Denmark); while, the nitrogen content was determined by using a nitrogen analyzer (Rapid N cube, Elementar, Hanau, Germany) and then converted into protein content using the N×6.25 equation (N = nitrogen content obtained from the samples, and 6.25 = conversion factor). To determine calories, the sample was homogenized in a blender then the homogenate was used for measurement of calorie content with a calorimeter (model 6400, Parr instrument, Moline, IL, USA). Calories were expressed as kcal/100 g of the sample.

pH and water activity (aw)

The pH values of samples were determined in triplicates using a pH meter (Model 340, Mettler-Toledo GmbH, Schwerzenbach, Switzerland) that was calibrated with 3 different standard pH solutions (4.0, 7.0, and 9.25). The pH was measured after homogenizing 3 g of each sample with 27 mL of distilled water for 30 s using a homogenizer.
Water activity (aw) of the fermented sausages was determined at 25°C with a measuring instrument; model AW SPRINT-TH 300 (Novasina Co, Lachen, Switzerland). Calibration was done by using several saturated solutions of known aw.

Color measurement

Color was determined at 3 defined areas on the cut surface of each sample using a Minolta Chroma Meter CR-400 (Minolta Camera Co., Ltd., Osaka, Japan) that was standardized with a white plate (Y = 86.3, X = 0.3165, and y = 0.3242). Special care was taken to avoid scanning areas of fat in the sample. Color was expressed according to the Commission International de l’Eclairage (CIE) system and reported as CIE L* (lightness), CIE a* (redness), CIE b* (yellowness), chroma and hue angle (h°). In which the chroma and hue angle were calculated as (a*2+b*2)0.5 and tan−1 (b*/a*), respectively.

Texture analysis

The texture properties were analyzed using a puncture probe (7 mm diameter) attached to a texture Analyzer (Model 4465, Instron Corp, High Wycombe, UK). For texture analysis, the samples from each treatment were cut into 2.5 cm long pieces; the cube was axially compressed twice until at each time reaching 80% of its initial height. The speed of load cell was set at 120 mm/min and the following parameters were calculated: hardness (kg), cohesiveness (kg×mm), gumminess (kg) and chewiness (kg×mm).

Lipid oxidation

The content of thiobarbituric acid reactive substances (TBARS) was determined to evaluate the lipid oxidation level in samples between the treatments, using the method of Pikul et al. (1987). Briefly, each sample (5 g) with 17.5 mL of 4% perchloric acid and 0.5 mL of 7.5% butylated hydroxyansole in ethanol was homogenized at 13,000×rpm for 20 s using a homogenizer (Polytron MR-2100, Kinematica AG, Lucerne, Switzerland). The volume of the homogenate was adjusted to 25 mL with 4% perchloric acid solution and then filtered through No. 1 Whatman filter paper. The filtrate (5 mL each) was transferred to separate test tubes and mixed with 5 mL of 0.02 M thiobarbituric acid solution, the mixtures were then heated at 80°C for 1 h in a water-bath. After being cooled with cold water, about 1.5 mL of each sample was taken and the absorbance was measured at 532 nm using an UV–visible spectrophotometer (Proteome Lab Du-800, Beckman Coulter, Inc., Fullerton, CA, USA). TBARS concentrations were calculated by multiplying the absorbance by a constant coefficient of 5.5 which was obtained from standard curves and known dilutions (0.1 to 0.8 μM) of 1,1,3,3 tetramethoxypropane (Aldrich, Louis, MA, USA). The TBARS value was expressed as mg malonaldehyde/kg of sample. Three repetitions were applied for each sample in each treatment.

Cholesterol, monosaccharide, and sodium compositions

The cholesterol, monosaccharide and sodium contents in the fermented sausages were determined using the methods standardized by the Ministry of Food and Drug Safety of Korea (MFDA, 2014).

Sensory evaluation

Sensory evaluation was performed using a panel consisting of 12 assessors. The panelists, with average age of 35 years, were the staff of the department of animal products processing and utilization, National Institute of Animal Science, Korea, and were chosen on the basis of previous experience in sensory evaluation of meat products. Briefly, each sausage sample was cut into 0.3 cm thick pieces, placed onto dishes and coded with random numbers. The panelists were seated in private seats under fluorescent lighting and were served with the sensory samples in a random manner. Five major sensory traits including aroma, texture, color, taste and overall acceptability were used and the samples were then evaluated for the aforementioned traits using a 7-point scale (1 point = extremely undesirable, 7 point = extremely desirable) as described by Meilgaard et al. (1991). The panelists were asked to refresh their mouth with drinking distilled water and salt-free crackers between samples. All sensory sessions were carried out in the sensory panel booth room equipped with white lighting at a constant temperature of about 20°C.

Statistic analysis

The data were subjected to statistical analysis using the Statistic Analysis System (SAS, 2007) package (SAS Institute, Cary, NC, USA). All data were analyzed by the general linear model procedure considering treatment the main effect. Means were compared using Duncan’s Multiple Range Test, with a significance of p<0.05.


Effect on proximate composition

The incorporation of seasonings into the processing formulations had minor effects on the proximate compositions (Table 1). For moisture, no differences were found between the control and treatments (p>0.05), however, when comparing the moisture contents among the treatments, the samples made with GHP (T4) had a significantly higher level than those made with doenjang (p<0.05). Moisture content is one of the important water-related criteria used to indicate the shelf-life stability of the dry cured meat products. The moisture contents ranging between the treatments from 34.90% to 38.36% in the present work were generally lower than the levels (41.3% to 54.7%) in fermented sausages ripened for 21 days (Kim et al., 2014), but slightly higher than the levels (29.32% to 33.93%) in fermented sausages ripened for 25 days (Yim et al., 2015). This is probably due to the differences in the added level of fat, casing size and ripening conditions among the studies. The samples made with GHP (T4) had lower fat content (26.38%) compared to those of the control as well as other remaining treatments (p<0.05). The protein contents ranged between the treatments from 23.06% to 25.78%, despite the addition of doenjang or gochu-ang resulting in approximately 1% protein increase, the statistically analytic results showed no differences between the control and these treatments (p>0.05). Comparing the protein contents between the treatments shows that the sausages made with gochu-jang (T2) and GHP (T4) had significantly higher levels compared to the ones made with GMP (T3) and GG (T5) (p<0.05). Regarding calories, the sausages made with gochu-jang (T2) had higher level compared to that of the control and other remaining treatments, probably due to the presence of sugar and sweet rice flour in this seasoning whereas, the lower energy found in sausages made with GHP (T4) could be due its lower fat content (Table 1).

Effect on technological quality traits

Some technological quality traits of the fermented sausages in different treatments are presented in Table 2. The pH values ranged between the treatments from 4.70 to 4.86, in which the sausages made with either doenjang or gochu-jang and garlic had significantly lower values in comparison with the control, this could be due to higher level of acid lactic produced in these treated samples. The pH values in the treated samples in present study were generally lower than the values (4.98 to 5.33) reported for the ones made with addition of starter cultures (Corrall et al., 2014; Yim et al., 2015). It is well known that the shelf-life of meat products is affected by a number of factors including pH condition; the lower the pH the higher the acidity and most bacteria that contribute to food spoilage do not prosper in acidic conditions. Thus, these pH results could be considered high enough to ensure the safety of meat products (Papadima and Bloukas, 1999), and also suggests that the incorporation of these seasonings may partly enhance the shelf-life stability of fermented sausages.
Statistical analysis revealed that the water activity (aw) in all treatments was not significantly different from the control (p>0.05), suggesting that the treatment did not affect the rate of water activity reduction. However, comparison of the aw values in samples between the treatments shows that the treatment with doenjang had significantly (p<0.05) lower value compared to the other remaining treatments. It is well known that the shelf-life stability of food products usually increases with decreasing aw, as a high aw (e.g., around 0.95) can form an ideal medium for bacterial growth (Frenandez-Salguero et al., 1993). The aw values in fermented sausages made with seasoning in the present study were lower than the values (0.906 to 0.916) in fermented sausages ripened for 61 days (Corral et al., 2014) but almost similar to the values (0.865) in samples ripened for 15 days (Zapenela et al., 1999). For weight loss, no differences occurred between the control and gochu-jang or GMP treatment (p>0.05), while the treatment with GHP had significantly higher while the treatment with garlic had lower loss levels compared to the control (p<0.05).
Regarding the lipid oxidation, it was observed that most treatments with the seasonings generally led to a reduction in TBARS content as compared to the control, however, the significant (p<0.05) differences only were observed for the samples treated with GHP or garlic. The results indicate that the lipid oxidation was inhibited by the added GHP and garlic contents; this could be due to the actions of antioxidants present in these seasonings. Earlier workers have shown that garlic and hot peppers contain bioactive compounds such as; allyl disulfide, alliin and allicin and phenols that are powerful antioxidants (Materska and Perucka, 2005; Chung, 2006). For the cases of doenjang, and gochu-jang treatments, despite these seasonings alone have been proven to possess high antioxidant activity in vitro and in vivo studies (Kim et al., 2005; Shukla et al., 2014), the addition of these contents at level of 1% did not significantly reduce the lipid oxidation in the fermented sausages, perhaps due to the low added level. Therefore, further study using higher concentrations is needed to assess the antioxidant potential of these seasonings in fermented sausages.

Effects on color

Color attributes of fermented sausages as affected by the addition of various seasonings are presented in Table 3. Regarding L* (lightness), all the treatments with seasonings did not affect the lightness of the products, except the treatment with GHP which showed lower L*-value in comparison to the control samples. Similarly, no differences in a*(redness)-values occurred between the control samples and those with added doenjang or gochu-jang and GHP (p>0.05). However, the sausages produced with gochu-jang had significantly (p<0.05) higher a*-values compared with that of the control, probably resulting from the suppression of the product’s natural color owing to the addition of gochu-jang. For the other remaining color traits such as b* (yellowness), chroma and hue angle, the values in most treatments were similar to that in the control samples, excepted for the treatment with gochu-jang which showed significantly (p<0.05) higher values. Color is the single most important factor of meat products affecting the consumer purchasing decision, and nitrite has long been used to enhance the red color of these products (Honikel, 2008). However, the use of nitrite in meat product is also associated with health risk (Chow and Hong, 2002), researchers therefore have been attempting to reduce the added nitrite content in meat products by partially replacing it with natural additives (Deda et al., 2007). Thus, it appears that the addition of the seasonings did not cause color defects in the fermented sausages. Additionally, the incorporation of gochu-jang improved the red color of fermented sausages, suggesting that the addition of gochu-jang to fermented sausages may be an alternative way to eliminate or reduce the level of nitrate.

Effect on texture

Table 4 presents the texture profiles of fermented sausages in different treatments. It was observed that that the addition of seasonings did not affect the texture parameters such as cohesiveness, gumminess and chewiness (p>0.05). Only the fermented sausages made with GMP had significantly (p<0.05) lower hardness value as compared to that of the control. These results suggest that the incorporation of seasonings did not cause a texture defect in the product.

Effect on cholesterol, monosaccharide and sodium contents

Table 5 presents the cholesterol, monosaccharide and sodium contents of fermented sausages made with/without test seasonings. The outcome of our analysis showed that the addition of the seasonings had beneficial effects on reduction of cholesterol content in fermented sausages. Particularly, the addition of doenjang or GMP, GHP and garlic resulted in reduction of 26.50, 32.54, 47.04, and 48.54 mg cholesterol/100 g samples, respectively as compared with that of the control (p<0.05). As previously mentioned in the introduction section, fermented sausages also have some negative aspects such as the high cholesterol level which is a main cause of cardiovascular disorder disease, high blood pressure and mortality. When compared to the cholesterol levels (110.5 mg/100 g sample) reported for fermented sausages in literature (Muguerza et al., 2001), all of the fermented sausages made with seasonings in the present study had lower levels, whereas those made without the test seasonings (control) had similar or higher level. Additionally, studies with human subjects have shown that consumption of either soya products or garlic, soluble fiber and plant sterol esters for several months significantly reduced serum concentrations of total cholesterol, low-density lipoproteins cholesterol and triglycerides (Hermansen et al., 2003), however, the possible mechanism of cholesterol-lowering effect of plant-sourced products in these studies still remains unclear. The present work, we assessed the effect of the addition of some traditional/natural seasonings on cholesterol content in meat products. Despite the exact mechanism underlying the cholesterol-reducing effect of the added seasonings remaining unknown, it is probably due to the bioactive components or enzymes originating from microorganisms present in these seasonings which degraded the cholesterol content in the product. Regarding this, Lee et al. (1997) and colleagues demonstrated that some microorganisms are capable of degrading cholesterol or β-sitosterol. From the results/observations of our investigation it could be concluded that the addition of natural/traditional seasonings to the conventional processing formulations have powerful effects on reduction of cholesterol content in fermented sausages.
For the monosaccharide and sodium contents, the results showed that no significant differences in their levels occurred between the treatment and control samples (p>0.05).

Effect on sensory traits

The effect of the addition of seasonings on the sensory traits of fermented sausages is shown in Table 6. In general, incorporating the seasonings had positive effects on improvement of sensory quality of the products; however, the effects differed depending on type of seasonings added and sensory traits evaluated. In particular, the fermented sausages made with either GMP or GHP and garlic had significantly (p<0.05) higher aroma and taste scores compared with that of the control. The rest of treatments with doenjang or gochu-jang also produced higher aroma and taste scores compared with that of the control but not significantly different (p>0.05). The reason the panelists gave higher taste and aroma scores for the fermented sausages made with the seasonings could be due to the contribution of the flavor enhancers and taste active compounds that exist within these seasonings. Additionally, the seasonings, especially chili peppers and garlic, have widely been used to enhance the flavor of many dishes for centuries due to their abundance and inexpensiveness. In meat industry, some of these ingredients are also used in meat products (Deda et al., 2007; Lorenzo et al., 2013), however, most of them are added to the meat products in the form of dry powder which has undergone a processing treatment that reduces their typical flavors. Similarly, all treatments (excepted for the case of doenjang) showed significantly higher color scores compared to that of the control. Overall acceptance is the sum of all sensory traits. Our results showed that all the treatments had generally higher acceptability scores than the control, however, statistically significant differences only were observed for the cases of doenjang, GMP and garlic treatments. The higher acceptability scores given by the panelists for these treated samples could be associated with the synergistic effect of their higher aroma, taste and color scores. Similarly, previous studies have also showed that the addition of natural ingredients (e.g., mushroom, tomato paste) had positive effects on the sensory quality of sausages (Dade et al., 2007; Seong et al., 2015). Hence our results suggest that there is the potential for these natural seasonings to improve the sensory quality of meat products in general and fermented sausages in particular.
In conclusion, the present work, for the first time assessed the impacts of addition of some traditional seasonings on the technological quality, cholesterol and sensory characteristics of fermented sausages. The addition of seasonings did not cause color or texture defects whereas they had beneficial effects on pH and lipid oxidation reductions in the final products. Noticeably, the addition of seasonings such as doenjang, medium-hot peppers, hot peppers and garlic significantly reduced the total cholesterol content in the products. Futhermore, the addition of these seasonings also had positive effects on improving sensory quality such as aroma, taste color and acceptability scores of the fermented sausages. Based on the results obtained in the present study it is clearly suggested that the test seasonings could be added as functional ingredients for reducing cholesterol content and improving lipid oxidation stability as well as sensory quality of fermented sausages.


This work was supported by “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ906974)” Rural Development Administration, Republic of Korea.



We certify that there is no conflict of interest with any financial organization regarding the material discussed in the manuscript.

Table 1
Proximate compositions of fermented sausages as affected by the seasonings addition1
Treatment Moisture (%) Fat (%) Calorie (Kcal/100 g) Protein (%)
Control (C) 37.28±1.37ab 27.38±0.65a 409.26±9.37b 24.70±1.17abc
1%doenjang (T1) 34.90±0.13b 28.58±0.47a 415.96±8.01b 25.46±0.29ab
1%gochu-jang (T2) 36.82±0.33ab 27.92±0.43a 440.50±6.66a 25.78±0.83a
1% ground medium-hot peppers (T3) 37.33±0.35ab 29.11±0.36a 411.66±6.81b 23.06±0.77c
1% ground hot peppers (T4) 38.36±0.59a 26.38±0.40b 376.56±11.95c 25.78±0.34a
1% ground garlic (T5) 37.35±1.81ab 27.75±0.77a 406.62±4.28b 23.34±0.23bc

1 Data are the means±standard deviation.

Values with different letters (a–c) in the same column differ significantly (p<0.05).

Table 2
Technological quality traits and lipid oxidation of fermented sausages as affected by the seasonings addition1
Treatment pH Water activity (aw) Weight loss (%) TBARS (mg MDA/kg sample)
Control (C) 4.86±0.01a 0.86±0.00ab 36.94±0.14c 1.53±0.2ab
1% doenjang (T1) 4.74±0.01b 0.86±0.00b 37.98±0.19b 1.30±0.1bc
1% gochu-jang (T2) 4.70±0.02b 0.86±0.00ab 37.20±0.14c 1.34±0.2bc
1% ground medium-hot peppers (T3) 4.75±0.01b 0.87±0.00a 37.03±0.21c 1.70±0.2a
1% ground hot peppers (T4) 4.85±0.02a 0.86±0.00a 40.73±0.26a 1.21±0.1c
1% ground garlic (T5) 4.73±0.02b 0.87±0.00a 35.36±0.41d 1.22±0.1c

TBARS, thiobarbituric acid reactive substances; MDA, malonaldehyde.

1 Data are the means±standard deviation.

Values with different letters (a–d) in the same column differ significantly (p<0.05).

Table 3
Color traits of fermented sausages as affected by the seasonings addition1
Treatment CIE L* CIE a* CIE b* Chroma Hue
Control (C) 54.34±0.57a 12.12±0.30bc 6.15±0.21b 13.60±0.36b 26.89±0.24d
1% doenjang (T1) 53.90±0.41a 12.47±0.35ab 6.07±0.14b 13.88±0.37b 25.99±0.26d
1% gochu-jang (T2) 53.92±0.87a 13.51±0.73a 10.09±0.61a 16.87±0.94a 36.67±0.46a
1% ground medium-hot peppers (T3) 54.13±0.36a 10.95±0.22c 6.96±0.11b 13.00±0.24b 32.60±0.12b
1% ground hot peppers (T4) 51.65±0.42b 12.74±0.31ab 6.24±0.10b 14.19±0.31b 26.11±0.36d
1% ground garlic (T5) 54.90±0.44a 11.61±0.22bc 6.48±0.12b 13.31±0.15b 29.23±0.86c

CIE, Commission International de l’Eclairage.

1 Data are the means±standard deviation.

Values with different letters (a–d) in the same column differ significantly (p<0.05).

Table 4
Texture profiles of fermented sausages as affected by adding traditional seasonings1
Treatment Hardness (kg) Cohesiveness Gumminess (kg) Chewiness (kg×mm)
Control (C) 1.83±0.19ab 1.29±0.34 2.42±0.77 7.03±4.22
1% doenjang (T1) 1.97±0.01a 1.33±0.35 2.62±0.71 7.88±3.26
1% gochu-jang (T2) 1.71±0.06ab 1.68±0.53 2.90±0.94 10.16±4.79
1% ground medium-hot peppers (T3) 1.33±0.06c 0.72±0.20 0.97±0.29 1.11±0.63
1% ground hot peppers (T4) 1.85±0.04ab 1.74±0.22 3.24±0.49 11.01±3.33
1% ground garlic (T5) 1.60±0.07bc 1.53±0.37 2.46±0.62 6.84±2.72

1 Data are the means±standard deviation.

Values with different letters (a–c) in the same column differ significantly (p<0.05).

Table 5
Cholesterol, monosaccharide and sodium contents of fermented sausages as affected by the seasonings addition1
Treatment Total cholesterol (mg/100 g) Monosaccharide (g/100 g) Na (mg/100 g)
Control (C) 118.66±13.45a 0.50±0.00ab 793.06±34.72ab
1% doenjang (T1) 92.12±5.31bc 0.50±0.00ab 984.18±100.06a
1% gochu-jang (T2) 104.10±5.59ab 0.46±0.02b 780.00±86.43ab
1% ground medium-hot peppers (T3) 86.12±6.92bc 0.46±0.02b 602.36±123.31b
1% ground hot peppers (T4) 71.62±7.24c 0.52±0.02a 875.06±67.21ab
1% ground garlic (T5) 70.12±2.70c 0.48±0.02ab 685.04±93.93b

1 Data are the means±standard deviation.

Values with different letters (a–c) in the same column differ significantly (p<0.05).

Table 6
Sensory characteristics of fermented sausages as affected by the seasonings addition1
Treatment Aroma Taste Color Acceptability
Control (C) 4.63±0.16c 5.04±0.18c 4.75±0.17b 4.84±0.15c
1% doenjang (T1) 5.00±0.07bc 5.46±0.08bc 5.17±0.15ab 5.38±0.14b
1% gochu-jang (T2) 5.00±0.24bc 5.50±0.28bc 5.42±0.21a 5.25±0.31bc
1% ground medium-hot peppers (T3) 5.34±0.12ab 5.59±0.05b 4.92±0.05b 5.37±0.04bc
1% ground hot peppers (T4) 5.30±0.13ab 5.38±0.14b 5.38±0.08a 5.46±0.16ab
1% ground garlic (T5) 5.71±0.25a 6.13±0.08a 5.42±0.11a 5.96±0.14a

1 Data are the means±standard deviation.

Values with different letters (a–c) in the same column differ significantly (p<0.05).


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