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Asian-Australas J Anim Sci > Volume 24(1); 2011 > Article
Invited Review
Asian-Australasian Journal of Animal Sciences 2011;24(1): 1-8.
DOI: https://doi.org/10.5713/ajas.2011.r.01    Published online December 21, 2010.
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells
Satoshi Sugimura, Eimei Sato
Abstract
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In canines, however, it is difficult to obtain large quantities of oocytes because each donor produces a limited number of mature oocytes in vivo. Although in vitro maturation (IVM) is considered an alternative approach to oocyte production, this technique is still too rudimentary to be used for the production of high-quality, uniform oocytes in large quantities. One technique for overcoming this difficulty is to use oocytes obtained from different species. This technique is known as interspecies SCNT (iSCNT). This review provides an overview of recent advances in canine -porcine interspecies SCNT.
Keywords: Canine; Interspecies; Porcine; Somatic cell Nuclear Transfer


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