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Asian-Australas J Anim Sci > Volume 23(9); 2010 > Article
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2010;23(9): 1159-1165.
DOI: https://doi.org/10.5713/ajas.2010.90551    Published online August 20, 2010.
Cloning and Distribution of Facilitative Glucose Transporter 2 (SLC2A2) in Pigs
Jianjun Zuo, Zhiyi Huang, Aimin Zhi, Shigeng Zou, Xiangyan Zhou, Fawen Dai, Hui Ye, Dingyuan Feng
Abstract
Glucose is the main energy source for mammalian cells and its absorption is co-mediated by two different families of glucose transporters, sodium/glucose co-transporters (SGLTs) and facilitative glucose transporters (GLUTs). Here, we report the cloning and tissue distribution of porcine GLUT2. The GLUT2 was cloned by RACE and its cDNA was 2,051 bp long (GenBank accession no. EF140874). An AAATAA consensus sequence at nucleotide positions 1936-1941 was located upstream of the poly (A)+ tail. Open reading frame analysis suggested that porcine GLUT2 contained 524 amino acids, with molecular weight of 57 kDa. The amino acid sequence of porcine GLUT2 was 87% and 79.4% identical with human and mouse GLUT2, respectively. GLUT2 mRNA was detected at highest level in porcine liver, at moderate levels in the small intestine and kidney, and at low levels in the brain, lung, muscle and heart. In the small intestine, the highest level was in the jejunum. In conclusion, the mRNA expression of GLUT2 was not only differentially regulated by age, but also differentially distributed along the small intestine of piglets, which may be related to availability of different intestinal luminal substrate concentrations resulting from different food sources and digestibility.
Keywords: GLUT2; Molecular Cloning; Small Intestine; Pig


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