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Asian-Australas J Anim Sci > Volume 23(1); 2010 > Article
Animal Breeding and Genetics
Asian-Australasian Journal of Animal Sciences 2010;23(1): 7-12.
DOI: https://doi.org/10.5713/ajas.2010.60023    Published online December 22, 2009.
Molecular Characterization, Chromosomal Localizations, Expression Profile, and Association Analysis of the Porcine PECI Gene with Carcass Traits
H. Gao, B. Fan, M. J. Zhu, B. Liu
Abstract
The full-length cDNA of the porcine peroxisomal 3,2-enoyl-CoA isomerase (PECI) gene encodes a monofunctional peroxisomal 3,2-enoyl-CoA isomerase. Cloning and sequencing of the porcine PECI cDNA revealed the presence of an 1185-base pair open reading frame predicted to encode a 394-amino acid protein by the 5’ rapid amplification of cDNA ends (5’-RACE) and EST sequences. The porcine PECI gene was expressed in seven tissues (heart, liver, spleen, lung, kidney, skeletal muscle, fat) which was revealed by reverse transcriptase-polymerase chain reaction (RT-PCR). The porcine PECI was mapped to SSC71/2 p11-13 using the somatic cell hybrid panel (SCHP) and the radiation hybrid panel (RH) (LOD score 12.84). The data showed that PECI was closely linked to marker S0383. A C/T single nucleotide polymorphism in PECI exon 10 (3’-UTR) was detected as a PvuII PCR-RFLP. Association analysis in our experimental pig population showed that different genotypes of PECI gene were significantly associated with the Average Backfat thickness (ABF) (p<0.05) and Buttock backfat thickness (p<0.01).
Keywords: Full-length cDNA; RH Mapping; Significant Association; Backfat Thickness; PECI Gene; Pigs


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