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Animal Biotechnology
Asian-Australasian Journal of Animal Sciences 2009;22(6): 885-892.
DOI: https://doi.org/10.5713/ajas.2009.80472    Published online April 30, 2009.
Proteomic Approach Analysis of Mammary Membrane Proteins Expression Profiles in Holstein Cows
Yong-xin Yang, Sui-zhong Cao, Yong Zhang, Xing-xu Zhao*
Correspondence:  Xing-xu Zhao,
Abstract
To investigate host defense mechanisms for protecting the mammary gland from mastitis infection, the membrane fraction of mammary tissues from Holstein cows was purified by differential velocity centrifugation, and then the sodium dodecyl sulfate-polyacrylamid gel electrophoresis (SDS-PAGE) separated proteins were identified by ion trap mass spectrometer equipped with a Surveyor high performance liquid chromatography (HPLC) system. A total of 183 proteins were identified. Bioinformatics software was applied to analyse physicochemical characteristics of the identified proteins and to predict biochemical function. These data may provide valuable information to investigate the mechanisms of mammary gland milk secretion and infectious disease, and enable a clear identification of proteins and potential protein targets for therapies.
Keywords: Holstein Cows; Mammary Gland; Proteome; Bioinformatics Software


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