Go to Top Go to Bottom
Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2009;22(6): 781-787.
DOI: https://doi.org/10.5713/ajas.2009.80515    Published online April 30, 2009.
The Expression Patterns of Cdc25A, Cdc25B, Sox2 and Mnb in Central Nervous System in Early Chicken Embryos
Hui Zhang, Junhui Qin, Jingjing Cao, Nainan Hei, Chunsheng Xu, Ping Yang, Haili Liu, Xiaohong Chu, Huijun Bao, Qiusheng Chen*
Correspondence:  Qiusheng Chen,
Abstract
The sense and antisense digoxigenin-labeled RNA probes of four genes, Cdc25A, Cdc25B, Sox2 and Mnb, were produced by using SP6 and T7 RNA polymerases, respectively, and in vitro transcription. Expression patterns of the four genes were detected by in situ hybridization in HH (Hamburger and Hamilton) stage 10 chick embryos. In general, expression patterns of the four genes were similar. mRNA of the four genes was mostly restricted to the entire CNS (central nervous system). All were confined to an identical region, neural tube, neural groove and caudal neural plate, corresponding to the notochord or spinal cord, but there was some distinction in specific region or in concentration, for example in somites. The overlap in expression at the same developmental stage in the CNS suggests that the four genes may be functional similar or related in CNS development. Expression patterns of the four genes support specific roles of these regulators in the developing CNS.
Keywords: Cdc25A; Cdc25B; Sox2; Mnb; CNS; In situ Hybridization; Chicken
TOOLS
METRICS Graph View
  • 0 Crossref
  •  0 Scopus
  • 1,975 View
  • 4 Download
Related articles


Editorial Office
Asian-Australasian Association of Animal Production Societies(AAAP)
Room 708 Sammo Sporex, 23, Sillim-ro 59-gil, Gwanak-gu, Seoul 08776, Korea   
TEL : +82-2-888-6558    FAX : +82-2-888-6559   
E-mail : jongkha@hotmail.com               

Copyright © 2020 by Asian-Australasian Journal of Animal Sciences. All rights reserved.

Close layer
prev next