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Asian-Australas J Anim Sci > Volume 22(5); 2009 > Article
Animal Biotechnology
Asian-Australasian Journal of Animal Sciences 2009;22(5): 712-720.
DOI: https://doi.org/10.5713/ajas.2009.80469    Published online April 30, 2009.
Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs
Shi-geng Zou, Ai-min Zhi, Xiang-yan Zhou, Jian-jun Zuo, Yan Zhang, Zhi-yi Huang, Ping-Wen Xu, Ding-yuan Feng*
Correspondence:  Ding-yuan Feng,
Abstract
The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcine cationic amino acid transporters and amino acid absorption. Porcine CAT-2 was cloned, sequenced and characterized. The predicted amino acid sequence of porcine CAT-2 shared 86.1% and 92.1% identity with human and mouse CAT-2A, respectively. The tissue distribution patterns and ontogenic changes of CAT-2 mRNAs were determined by real-time Q-PCR. The results showed that porcine CAT-2 was highly expressed in the heart and intestinal tract (duodenum, ileum and jejunum). In addition, the mRNA of CAT-2 was found in liver, lung, kidney, brain and muscle. Within the intestinal tract, CAT-2 mRNA was most abundant in the ileum and rarely expressed in the duodenum. In the duodenum, the levels of CAT-2 mRNA reached their peak on day 7 (p<0.05) while in the jejunum, levels were low on day 1 and 7 and increased rapidly after day 26 before peaking on days 30 and 60 (p<0.05). The levels then dramatically decreased by day 90 (p<0.05). In the ileum, levels achieved their maximum on day 30 and then decreased significantly on day 60 (p<0.05).
Keywords: Amino Acid Transporter; CAT-2; SLC7A2; Ontogenetic Regulation; Molecular Cloning; Pig


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