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Ruminant Nutrition and Forage Utilization
Asian-Australasian Journal of Animal Sciences 2009;22(2): 201-205.
DOI: https://doi.org/10.5713/ajas.2009.80579    Published online January 6, 2009.
Effects of Cordyceps militaris Mycelia on In vitro Rumen Microbial Fermentation
Joon Mo Yeo, Shin Ja Lee, Sang Min Lee, Sung Hwan Shin, Sung Hoon Lee, Jong K. Ha, WanYoung Kim, Sung Sill Lee*
Correspondence:  Sung Sill Lee,
Effects of Cordyceps militaris mycelia on rumen microbial fermentation were determined by measuring in vitro gas production, cellulose digestion and VFA concentrations. C. militaris mycelia was added to buffered rumen fluid with final concentrations of 0.00, 0.10, 0.15, 0.20, 0.25 and 0.30 g/L and incubation times were for 3, 6, 9, 12, 24, 36, 48 and 72 h. At all incubation times, the gas production showed a quadratic increase with the supplementation of C. militaris mycelia; maximum responses were seen with 0.25 g/L supplementation. However, the gas production was significantly lower for the 0.30 g/L supplementation than for the 0.25 g/L supplementation from 9 h to 72 h incubation. The cellulose filter paper (FP) digestion showed a quadratic increase, as did the gas production except at 3 h incubation. The concentration of total VFA was significantly increased by the supplementation of C. militaris mycelia compared with the control treatment; the highest response was also seen with 0.25 g/L supplementation. This was true for responses in the concentration of acetic and propionic acids. As opposed to other responses, the responses of pH to the supplementation of C. militaris mycelia showed a quadratic decrease from 3 h to 36 h incubation. In conclusion, C. militaris mycelia alter the mixed rumen microbial fermentation with increases in the production of gas and VFA, and cellulose FP digestion.
Keywords: Cordyceps militaris; Rumen Microorganisms; In vitro Gas Production

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