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Animal Reproduction and Physiology
Asian-Australasian Journal of Animal Sciences 2009;22(1): 35-41.
DOI: https://doi.org/10.5713/ajas.2009.80185    Published online January 6, 2009.
Effects of β-Mercaptoethanol on the Growth of Preantral Follicles and the Maturation of Intrafollicular Oocytes
Seung Pyo Gong, Jeong Mook Lim*
Correspondence:  Jeong Mook Lim,
Abstract
This study was undertaken to evaluate how -mercaptoethanol (bME), an exogenous antioxidant, interacts with preantral follicles cultured in vitro. Mouse primary or secondary follicles were cultured in glutathione (GSH)-free or GSH-containing medium supplemented with bME of various concentrations, and the growth of preantral follicles, the maturation of intrafollicular oocytes and preimplantation development after parthenogenesis were monitored. In experiment 1, 0, 25, 50 or 100 M bME was added to culture medium supplemented with 100 M GSH or not. When secondary follicles were cultured in GSH-free medium, no significant change in follicle growth was detected after bME addition. However, exposure to bME in the presence of GSH significantly inhibited both follicle growth and oocyte maturation. Such detrimental effect became prominent in primary follicles and bME strongly inhibited follicle growth in the absence of GSH. In conclusion, there are stage-dependent effects of bME on follicle growth and oocyte maturation, and selective use of antioxidants contributes to establishing an efficient follicle culture system.
Keywords: Preantral Follicle; In-vitro Culture; b-Mercaptoethanol; Follicle Growth; Oocyte Maturation


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